Transcriptomics

Dataset Information

0

Single Cell Transcriptomic Analysis of Prostate Cancer Cells


ABSTRACT: The ability to interrogate circulating tumor cells (CTC) and disseminated tumor cells (DTC) is restricted by the small number detected and isolated (typically <10). We wanted to determine if a commercially available technology could provide a transcriptomic profile of a single prostate cancer (PCa) cell. We clonally selected and cultured a single passage of cell cycle synchronized C4-2B PCa cells. Ten sets of single, 5-, or 10-cells were isolated using a micromanipulator under direct visualization with an inverted microscope. Additionally, we analyzed 10 individual DTC isolated from each of 2 patients with metastatic PCa. We have shown that a transcriptomic profile can be obtained from a single cell using commercially available technology. As expected, fewer amplified genes are detected from a single-cell sample than from pooled cell samples, but this method can be used to obtain a transcriptomic profile from DTC isolated from the bone marrow of patients with PCa.

ORGANISM(S): Homo sapiens

PROVIDER: GSE38416 | GEO | 2013/02/19

SECONDARY ACCESSION(S): PRJNA168012

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-02-19 | E-GEOD-38416 | biostudies-arrayexpress
2014-09-16 | E-GEOD-48995 | biostudies-arrayexpress
2022-03-31 | E-MTAB-11557 | biostudies-arrayexpress
2014-09-16 | GSE48995 | GEO
2015-10-26 | E-GEOD-64262 | biostudies-arrayexpress
| PRJNA278079 | ENA
2024-10-01 | GSE274755 | GEO
2015-01-30 | E-GEOD-58700 | biostudies-arrayexpress
| PRJNA474334 | ENA
| PRJNA1089333 | ENA