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Gene expression from induced CB-derived neurons at different time of differentiation

ABSTRACT: Gene expression from cord blood stem cells and respective derived neuronal cells at different times point of differentiation:CD133+ cells; Keywords: Expression Profiling by array

ORGANISM(S): Homo sapiens

PROVIDER: GSE38431 | GEO | 2012/06/05

SECONDARY ACCESSION(S): PRJNA168013

REPOSITORIES: GEO

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Gene expression from induced CB-derived neurons at different time of differentiation

Project description:Gene expression from cord blood stem cells and respective derived neuronal cells at different times point of differentiation:CD133+ cells; Keywords: Expression Profiling by array We analyzed 3 arrays from each condition and 2 arrays for the positive controls:CB CD133+ untreated,CB-iNCs after SOX2 and cMYC overexpression, CB-neurons after differentiation (6 weeks), HUES6-NPCs control, HUES6-neurons control

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Project description:To demonstrate CD133+CD44+ and CD133+CD44- subpopulations of hepatocellular carcinoma as distinct subgroups, we have employed whole genome microarray expression profiling as a discovery platform to reveal the gene profiles of different subgroups and identify genes responsible for the enhanced metastatic potentials of CD133+CD44+ tumor cells. CD133+CD44+ and CD133+CD44- tumor cells were isolated from three human metastatic hepatocellular carcinoma specimens. A 76-gene consensus signature was identified that distinguished between CD133+CD44+ and CD133+CD44- subgroups. CD133+CD44+ and CD133+CD44- subgroups from different patients were well clustered as two distinct classes according to this signature, and many genes in this signature were reported involved in tumor metastasis. Expression of four genes (CCL4, DKK3, CCR5 and MMP12) from this signature was confirmed in another three metastatic HCC specimens by real-time PCR.

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