Transcriptomics

Dataset Information

0

Regulation of pluripotency and self-renewal of ES cells through epigenetic-threshold modulation and mRNA pruning


ABSTRACT: The core pluripotency factors (Oct4, Sox2, and Nanog), the Myc network, and the chromatin-modifying complexes such as PRC2 ensure the pluripotency and self-renewal of ES cells (ESC). How these factors coordinate with one another remains poorly understood. We report that Utf1, a target of Oct4 and Sox2, is a new bivalent chromatin component that buffers poised states of bivalent genes. By limiting PRC2 loading and Histone 3 lysine-27 trimethylation, Utf1 sets proper activation thresholds for bivalent genes. It also promotes nuclear tagging of mRNAs transcribed from insufficiently silenced bivalent genes for cytoplasmic degradation through mRNA de-capping. Whereas these opposing functions of Utf1 allow proper execution of ESC pluripotency, the mRNA pruning function also ensures rapid cell proliferation by blocking the Myc-Arf feedback regulation. Thus, Utf1 is an important regulator that couples the core pluripotency factors with Myc and PRC2 networks to promote proliferation and pluripotency execution of ESCs.

ORGANISM(S): Mus musculus

PROVIDER: GSE39513 | GEO | 2012/10/26

SECONDARY ACCESSION(S): PRJNA171100

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2012-10-26 | E-GEOD-39513 | biostudies-arrayexpress
2013-05-28 | E-GEOD-47442 | biostudies-arrayexpress
2013-05-28 | E-GEOD-47441 | biostudies-arrayexpress
2013-05-28 | GSE47441 | GEO
2013-05-28 | GSE47442 | GEO
2014-08-28 | E-GEOD-49305 | biostudies-arrayexpress
2014-08-28 | GSE49305 | GEO
2005-01-01 | MODEL1305010000 | BioModels
2012-02-20 | E-GEOD-34801 | biostudies-arrayexpress
2008-11-07 | GSE12922 | GEO