Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiles of adult neural stem cells with loss-of-function and gain-of-function of SIRT1

ABSTRACT: To elucidate the role of SIRT1 in adult neural stem cells, we have carried out a genome-wide microarray analysis on cultured adult neurospheres from wildtype (WT) and sirt1 knockout (KO) mice and WT neurospheres treated with DMSO and SIRT1 activator,resveratrol for 24 hours. Then we screened for genes that exhibited opposite changing patterns beween KO-versus-WT and resveratrol-versus-DMSO-treated groups. Our result revealed a significant enrichment of genes involved in metabolic process. And numerous genes involved in cell proliferation and differentiation were represented in these SIRT1-responsive genes. More interestingly, Notch signaling-related genes,such as Dll4, Mib1,Hes5, Heyl, Hey2 and so on, also showed a significant enrichment. Real-time PCR validated the expression of Notch signaling-related genes. These data revealed the regulation of SIRT1 on the self-renewal and differentiation of adult neural stem cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE39551 | GEO | 2015/03/30

SECONDARY ACCESSION(S): PRJNA171112

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene profile beween CD19 normal and deletion of resting B cells in spleen of sirt1 conditional knockout mouse

Project description:Gene profile beween CD19 normal and deletion of resting B cells in spleen of sirt1 conditional knockout mouse

2019-05-01 | GSE121306 | GEO

The NOTCH3 Downstream Target HEYL Is Required for Efficient Human Airway Basal Cell Differentiation [scRNA-Seq]

Project description:Basal cells (BC) are the resident stem/progenitor cells of the adult pseudostratified airway epithelium, whose differentiation program is orchestrated by the NOTCH signaling pathway. NOTCH3 receptor mediated signaling regulates BC to club cell differentiation; however, the downstream responses that regulate this process are largely unknown. In the present study we utilized an in vitro air-liquid interface model of the human pseudostratified airway epithelium to identify the NOTCH3-dependent downstream genes/pathways that regulate human BC to club cell differentiation. Activation of NOTCH3 signaling in BC via lentivirus-mediated over-expression of the active NOTCH3 intracellular domain (NICD3) promoted club cell differentiation. Bulk RNA-seq analysis of control vs NICD3 -transduced cells, identified 692 NICD3 responsive genes enriched for pathways linked to airway epithelial biology and differentiation including Wnt/β-catenin Signaling. Expression of the classical NOTCH target HEYL increased in response to NOTCH3 activation and positively correlated with the club cell marker SCGB1A1. Further, using single-cell RNA-seq, we report that HEYL+ cells primarily clustered with SCGB1A1+ and NOTCH3+ cells. Moreover, HEYL protein co-localized with SCGB1A1 in ALI cultures in vitro and in the human and mouse airway epithelium in vivo. siRNA-mediated knockdown of HEYL in BC led to changes in epithelial structure including altered morphology and significant reductions in transepithelial electrical resistance and expression of tight junction related genes. Finally, HEYL knockdown significantly reduced the number of SCGB1A1+ club cells, along with a corresponding increase in KRT8+ BC-intermediate cells. Overall, our data identifies NOTCH3-HEYL signaling as a key regulator of BC to club cell differentiation.

2021-03-04 | GSE168127 | GEO

The NOTCH3 Downstream Target HEYL Is Required for Efficient Human Airway Basal Cell Differentiation [RNA-Seq]

2021-03-04 | GSE168126 | GEO

Expression data from isogenic Pten WT or KO mouse T-ALLs treated with DBZ or DMSO

Project description:To investigate the underlying mechanisms mediating resistance to NOTCH inhibition in Pten-null T-ALL tumor cells we performed gene expression profiling of isogenic Pten-positive and Pten-deleted leukemia lymphoblasts after acute treatment with DBZ in vivo. This analysis revealed that, while direct NOTCH1 target genes (such as Hes1, Dtx1, PtcrA, HeyL and Notch3) are effectively downregulated in both Pten-positive and Pten-deleted tumors, genetic ablation of Pten elicits a global reversal of much of the transcriptional effects of NOTCH inhibition. We performed microarray gene expression analysis of GSI treatment in isogenic Pten KO or WT NOTCH1 induced leukemias

2015-08-31 | E-GEOD-71087 | biostudies-arrayexpress

Gene expression analysis in lung fibroblasts from p38+/+ (wt) and p38ki/ki (ki) mice

Project description:To assess differently expressed genes beween wt and ki fibroblasts

2016-06-01 | GSE80825 | GEO

Convergence of SIRT1 and ATR signaling to modulate replication origin dormancy

Project description:SIRT1 chromatin interactome analysis was performed to identify SIRT1 molecular targets involved in replication initiation from cells containing either WT or H363Y SIRT1. Co-IP-MS of pT530 SIRT1 from chromatin extracts was performed. Files are: 1422 WT SIRT1 untreated U2OS; 1423 WT SIRT1 H2O2 treated, U2OS; 1424 H363Y SIRT1 untreated U2OS; 1425 H363Y SIRT1 H2O2 U2OS; 1426 KO SIRT1 untreated U2OS; 1427 KO SIRT1 H2O2 U2OS; 1428 WT SIRT1 untreated HEK; 1429 WT SIRT1 H2O2 HEK; 1430 WT SIRT1 untreated HCT116; 1431 WT SIRT1 H2O2 HCT116; 1432 WT SIRT1 untreated K562; 1433 WT SIRT1 H2O2 K562. Each sample has 9 fractions.

| MSV000088103 | MassIVE

Gene expression analysis in lung fibroblasts from p38+/+ (wt) and p38ki/ki (ki) mice

Project description:To assess differently expressed genes beween wt and ki fibroblasts Total RNA was purified from fibroblasts of 3 different mice for wt and 3 different mice for ki

2016-06-01 | E-GEOD-80825 | biostudies-arrayexpress

Plant-derived compounds strigolactone GR24 and pinosylvin modulate gene expression in rat L6 skeletal muscle cells

Project description:We investigated the effects of novel SIRT1 activating plant-derived compounds strigolactone GR24 and pinosylvin on gene expression in rat L6 skeletal muscle myotubes. Resveratrol was included in analyses as a reference compound.

2017-12-18 | GSE90833 | GEO

Expression data from isogenic Pten WT or KO mouse T-ALLs treated with DBZ or DMSO

2015-08-31 | GSE71087 | GEO

Effects of TET3 deletion on gene expression in ileum [RNA-seq]

Project description:Bulk RNA-seq show differential gene expression profile beween Wild type (WT) and Tet3 knockout (KO) in ileum. We identified genes involved in innate immune defense, anti-microbial response and growth signaling to be affected by TET3 deletion.

2023-09-04 | GSE241790 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data