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RpoHI and RpoHII regulons in Rhodobacter sphaeroides 2.4.1 from gene expression profiling

ABSTRACT: In this study, we performed expression profiling experiments to determine the respective regulons of RpoHI and RpoHII in Rhodobacter sphaeroides. We grew R. sphaeroides in aerobic conditions and induced either proteins ectopically and compared the global gene expression profiles.

ORGANISM(S): Cereibacter sphaeroides 2.4.1

PROVIDER: GSE39712 | GEO | 2012/08/03

SECONDARY ACCESSION(S): PRJNA171865

REPOSITORIES: GEO

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RpoHI and RpoHII regulons in Rhodobacter sphaeroides 2.4.1

Project description:This SuperSeries is composed of the following subset Series: GSE39711: RpoHI and RpoHII regulons in Rhodobacter sphaeroides 2.4.1 from chromatin immuno-precipitation GSE39712: RpoHI and RpoHII regulons in Rhodobacter sphaeroides 2.4.1 from gene expression profiling Refer to individual Series

2012-08-02 | E-GEOD-39806 | biostudies-arrayexpress

RpoHI and RpoHII regulons in Rhodobacter sphaeroides 2.4.1 from chromatin immuno-precipitation

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RpoHI and RpoHII regulons in Rhodobacter sphaeroides 2.4.1 from gene expression profiling

Project description:In this study, we performed expression profiling experiments to determine the respective regulons of RpoHI and RpoHII in Rhodobacter sphaeroides. We grew R. sphaeroides in aerobic conditions and induced either proteins ectopically and compared the global gene expression profiles. The expression profiles of R. sphaeroides strains lacking either RpoHI or RpoHII were compered to strains expressing RpoHI or RpoHII ectopically from a plasmid to detect gene differentially expressed. Three biological replicates were performed for each strain.

2012-08-02 | E-GEOD-39712 | biostudies-arrayexpress

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Project description:In this study, we performed a ChIP-chip experiment to determine the respective regulons of RpoHI and RpoHII in Rhodobacter sphaeroides. We grew R. sphaeroides in aerobic conditions and induced either proteins ectopically and immuno-precipitated the regions of the genomic DNA interacting with the sigma factors. DNA immunoprecipitated with antibodies against RpoHI or RpoHII was labelled with Cy5 and hybridized on two-color tilling arrays (triplicates for each) with genomic DNA as an input control labelled with Cy3.

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