Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Difference in expression between SA- and mock-treated leaves 2 hours after application

ABSTRACT: Arabidopsis plants, ecotype Columbia, were sprayed with surfactant alone (0.01% Silwet) or surfactant and 1mM salicylic acid until all leaves were wet, 3 to 4 weeks after germination (before flowering). Leaves were harvested and frozen in liquid nitrogen. Three biological replicates were obtained over a period of 6 months. For each replicate, RNA was extracted using standard phenol/chloroform protocol and biotin-labeled cRNA was synthesized. cRNA was hybridized to 6 ATH1 GeneChip arrays. Cel files produced by the Affymetrix software were imported into R. Background subtraction, normalization and probe summaries were performed with the rma, quantile.normalization and median polish options of the rma function. Comparison of the SA- versus mock-treated tissue provides insight on the genes involved in systemic acquired resistance in Arabidopsis. Keywords: salicylic acid, Arabidopsis, stress response, defense,

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE3984 | GEO | 2006/03/30

SECONDARY ACCESSION(S): PRJNA94251

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of Arabidopsis leaf samples treated with salicylic acid

Project description:Arabidopsis plants, ecotype Columbia, were sprayed with surfactant alone (0.01% Silwet) or surfactant and 1mM salicylic acid until all leaves were wet, 3 to 4 weeks after germination (before flowering). Leaves were harvested and frozen in liquid nitrogen. Three biological replicates were obtained over a period of 6 months. For each replicate, RNA was extracted using standard phenol/chloroform protocol and biotin-labeled cRNA was synthesized. cRNA was hybridized to 6 ATH1 GeneChip arrays. Cel files produced by the Affymetrix software were imported into R. Background subtraction, normalization and probe summaries were performed with the rma, quantile.normalization and median polish options of the rma function. Comparison of the SA- versus mock-treated tissue provides insight on the genes involved in systemic acquired resistance in Arabidopsis. Experiment Overall Design: 3 mock-treated and 3 SA-treated biological replicates were analyzed (one array each)

2007-10-04 | E-GEOD-3984 | biostudies-arrayexpress

Exogenous slaicylic acid (SA) treatment of Arabidopsis thaliana Col-0

2011-11-03 | GSE33402 | GEO

Exogenous slaicylic acid (SA) treatment of Arabidopsis thaliana Col-0

Project description:0.5 mM SA plus 0.02% Silwet or 0.02% Silwet (control) was sprayed on leaves of 3.5 week old Arabidopsis plants. Samples were harvested at 0 (prior to treatment) , 3, 6, 12, and 24 hours post treatment. A subset of these samples were processed. Arabidopsis plants grown in parallel under standardized conditions were treated with SA + Silwet or Silwet alone (control). Only mature leaves of the same developmental age were harvested using leaves from 2-4 plants, totalling ~0.2 grams per sample. Plants were not resampled. In our hands, experimental replicates are highly reproducible. This was an exploratory experiment to look for candidate genes impacted by exogenous SA treatment. We were only able to process a subset of samples and chose to process key time points, sacrificing replicates at each time point.

2011-11-03 | E-GEOD-33402 | biostudies-arrayexpress

Salicylic acid treatment and its effect on seed yield and seed molecular composition of Pisum sativum under abiotic stress

Project description:Pisum sativum (cv. Kudrnac) seeds were obtained from SEMO a.s., Smržice, Czechia. All plants used for the study grew in the experimental field plot at Veletiny, Czechia (49°02'N 17°33'E; 195 m a.s.l.). Sowing was done after the rainfall on June 1, 2022, and plants were grown in a natural environment without any additional treatment. After 35 days, the field was divided into three blocks (mock, SAt1, SAt2), each containing approxi-mately 100 plants. For five weeks, plants were weekly sprayed with 1.5 L water solution supplemented with 0.025% (v/v) Silwet L-77 (mock; K), or 100 µM salicylic acid and Silwet (final concentration as for the mock; SA-a). The third set was treated as the mock for two weeks, followed by four weeks with salicylic acid treatment (SA-b).

2023-03-17 | PXD040506 | Pride

Defense signaling in potato

Project description:Potato cultivar Russet Norkotah was grown in a growth chamber at 16 hour light/8 hour dark at 22°C fertilized with Osmocote. Plants were from tissue culture, transplanted to soil and used approximately 4 weeks after transplanting. RNA was extracted from leaves using Trizol. Plants were sprayed with one of four treatments, either 1mM salicylic acid, 100 µM methyl jasmonate, 1 mg/ml arachidonic acid or 100 µM methyl jasmonate plus 1 mM salicylic acid together. Samples were harvested at 3, 24 and 72 hours after spraying. Each salicylic acid or arachidonic acid treatment has 3 biological replicates done in parallel, using leaves pooled from 2 plants. The control for SA or AA treated plants were water sprayed plants, from which leaves of 3 different plants were pooled at each timepoint, done side-by-side with the treated samples. Methyl jasmonate or MJ+SA treatments had 2 biological replicates each, using leaves pooled from 2 plants. A separate set of water sprayed plants (from the SA and AA control treatments) were used as controls, and pooled leaves from 3 plants were used at each time-point, and done in parallel with the treated samples. Keywords: Direct comparison

2007-09-01 | GSE8208 | GEO

Transcription profiling of Arabidposis Col-0 plants sprayed with sodium nitroprusside (SNP)

Project description:22 day old Arabidopsis Col-0 plants were sprayed with 0.5 mM sodium nitroprusside (SNP), corresponding controls were sprayed with water, and leaves harvested 3 hours after spraying and frozen in liquid nitrogen.

2007-09-28 | E-MEXP-1268 | biostudies-arrayexpress

Gene expression of lpp2 KO mutant in response to ABA.

Project description:Transcriptomic study of lpp2 KO mutant in response to abcsisic acid - Fully developed rosette leaves were immersed for 1 min in a solution of 10-5 M ABA with 0.1% DMSO and 0.05%silwet. RNA are extracted after a 3h incubation. This treatment is compared to control (1 min immersion in 0.1% DMSO and 0.05%silwet) then 3h incubation. Keywords: treated vs untreated comparison

2009-07-01 | GSE14446 | GEO

G3P triggered changes in systemic transcriptome

Project description:Goal of this study was to determine changes in transcription profile in mock versus G3P treated plants. Arabidopsis (Col-0 ecotype) plants were infiltrated with petiole exudate, with or without G3P, and distal leaves were sampled 24 h post treatments. Keywords: Glycerol-3-Phosphate, Exudate, Salicylic acid, Arabidopsis, Systemic Acquired Resistance, Defense

2011-03-15 | GSE26973 | GEO

Exon-level summary data from Affymetrix Human Exon 1.0 ST array

Project description:The Affymetrix Human Exon 1.0 ST array was used to measure differential splicing patterns in archived RNA isolated from 26 of 80 children (11 Rejectors and 15 Non-Rejectors). The exon-level probe summaries reported in this series were computed using the Affymetrix Power Tools (APT) software and 'rma-sketch' normalization method. Keywords: Affymetrix 1.0 ST exon array; exon-level analysis

2008-09-15 | GSE11360 | GEO

Gene-level summary data from Affymetrix Human Exon 1.0 ST array

Project description:The Affymetrix Human Exon 1.0 ST array was used to measure differential splicing patterns in archived RNA isolated from 26 of 80 children (11 Rejectors and 15 Non-Rejectors). The gene-level probe summaries reported in this series were computed using the Affymetrix Power Tools (APT) software and 'rma-sketch' normalization method. Keywords: Affymetrix 1.0 ST exon array; gene-level analysis

2008-09-15 | GSE11359 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data