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A link between host plant adaptation and pesticide resistance in the polyphagous spider mite Tetranychus urticae

ABSTRACT: The goal of our microarray experiments was twofold: 1) Compare the gene expression profile of acaricide resistant spider mite strains (MAR-AB and MR-VP) with that of a susceptible spider mite strain (London); 2) Study gene expression changes in spider mites from the London strain upon transfer from bean, a suitable host, to tomato, a less favorable host. These gene expression changes upon host change were measured for three timepoints (2 hour on tomato (Tomato-2h), 12 hour on tomato (Tomato-12h) and 5 generations on tomato (Tomato-5G)).

ORGANISM(S): Tetranychus urticae

PROVIDER: GSE39869 | GEO | 2012/12/04

SECONDARY ACCESSION(S): PRJNA171920

REPOSITORIES: GEO

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Host plant shift and acaricide resistance effects on gene expression in the two-spotted spider mite, T. urticae

Project description:The goal of our microarray experiments was twofold: 1) Compare the gene expression profile of acaricide resistant spider mite strains (MAR-AB and MR-VP) with that of a susceptible spider mite strain (London); 2) Study gene expression changes in spider mites from the London strain upon transfer from bean, a suitable host, to tomato, a less favorable host. These gene expression changes upon host change were measured for three timepoints (2 hour on tomato (Tomato-2h), 12 hour on tomato (Tomato-12h) and 5 generations on tomato (Tomato-5G)). 23 samples were analyzed: 6 biological replicates for MR-VP, 5 biological replicates for MAR-AB and four biological replicates each for Tomato-2h, Tomato-12h and Tomato-5G

2012-12-04 | E-GEOD-39869 | biostudies-arrayexpress

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Whole genome tomato transcriptional response to Tetranychus urticae herbivory [Heinz1706]

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Cyenopyrafen resistance in the two-spotted spider mite Tetranychus urticae

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Whole genome tomato transcriptional response to jasmonic acid treatment and Tetranychus urticae herbivory [def-1]

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Tomato spotted wilt virus benefits a non-vector arthropod Tetranychus urticae by modulating different plant responses in tomato

Project description:Five-week old tomato plants were subjected to four treatments: 1) mock-inoculated, 2) virus-infected, 3) spider mite infestation, and 4) virus + spider mite infestation. Plant tissue was harvested from each treatment after 7-days, frozen in liquid nitrogen and stored in -80 degree Celsius until use.

2013-05-15 | E-MEXP-3888 | biostudies-arrayexpress

Tetranychus urticae secretome - The Salivary Protein Repertoire of the Polyphagous Spider Mite Tetranychus urticae: A Quest for Effectors

Project description:The two-spotted spider mite Tetranychus urticae is an extreme polyphaguous crop pest. Next to an increased detoxification potential of plant secondary metabolites, it has recently been shown that spider mites manipulate plant defences. Salivary constituents are proposed to play an important role during the interaction with its many hosts. The proteomic composition of saliva delivered into artificial diet by spider mites adapted to various hosts - bean, soy, maize, tomato -was determined using Orbitrap mass spectrometry. Over 200 different proteins were identified, many of unknown function and in numerous cases belonging to multi-membered gene families. A selection of these putative salivary proteins was validated using whole-mount in situ hybridizations and expression was shown to be localized in the anterior and dorsal podocephalic glands of the spider mite. Host-plant dependent expression was evident from the proteomics dataset and was further studied in detail by micro-array based genome wide gene expression profiling of mites maintained on the host plants under study. Previously obtained gene-expression datasets were further used to get more insight in the expression profile over different life stages and physiological states. To conclude, for the first time the T. urticae salivary proteome repertoire was characterized using a custom feeding hemisphere-based enrichment technique. This knowledge will assist in unraveling the molecular interactions between phytophagous mites and their host plants. This may ultimately facilitate the development of mite-resistant crops.

2017-01-17 | PXD003022 | Pride

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