Transcriptomics

Dataset Information

0

Gene expression profiles of tumor-positive sentinel lymph node biopsies from cutaneous melanoma patients


ABSTRACT: Sentinel lymph node, the first node draining the primary tumor, is a key component of tumor microenvironment promoting immune tolerance. In melanoma, sentinel node is one of the most important prognostic factors and the most frequent site of regional metastasis. To unravel the immunomodulatory pathways that are triggered by melanoma cells in the draining node that allow tumor spreading, transcriptional profiles associated to disease progression were analyzed in archival sentinel node biopsies (SNB). Gene expression profiles of melanoma-positive and negative SNB selected to maximize the differences in terms of disease stage and course, revealed subgroups correlating with regional node involvement and disease progression within positive biopsies. Transcriptional profiles revealed that genes showing differential expression between tumor-positive SNB with/without disease progression were mainly related to inflammatory response and that were mostly down regulated in patients with poor prognosis. TNFRSF8 encoding CD30 showing up regulation in SNB from patients with progressing disease displayed higher expression by immunohistochemical staining compared to SNB from non progressing patients. Subpopulations of CD30 positive CD4/CD8 double negative and CD4 Foxp3/PD-1 CD147 positive T cells were identified by flow cytometry analysis in metastatic nodes, suggesting a potential role of regulatory and tolerogenic T cells in melanoma progression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE39945 | GEO | 2013/08/01

SECONDARY ACCESSION(S): PRJNA172138

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-08-01 | E-GEOD-39945 | biostudies-arrayexpress
2017-01-31 | GSE88727 | GEO
2011-07-14 | E-GEOD-30688 | biostudies-arrayexpress
2018-10-26 | PXD005539 | Pride
2011-07-15 | GSE30688 | GEO
2021-07-16 | PXD018891 | Pride
2024-05-13 | GSE253592 | GEO
2024-05-13 | GSE253590 | GEO
2024-05-13 | GSE253589 | GEO
2024-05-13 | GSE253586 | GEO