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Heat shock - kin82 mutant and wildtype

ABSTRACT: Wild type and kin82 mutant under heat shock. Cells grown at 25°C were collected by centrifugation, resuspended in an equal volume of 37°C medium, and returned to 37°C for growth. Samples were collected at 0, 5, 15, 30 and 60 minutes after transfer to 37°C. Experimental samples were used to generate Cy5-labeled cDNA probes, whereas mRNA reference pools extracted from cultures of the respective strains grown to early log phase under normal conditions, were used to generate Cy3-labeled cDNA probes. Cy5- and Cy3-labeled probes were hybridized together to microarrays printed with PCR-amplified fragments, representing 6280 of the Saccharomyces cerevisiae ORFs. Keywords: time-course

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE406 | GEO | 2003/04/15

SECONDARY ACCESSION(S): PRJNA84953

REPOSITORIES: GEO

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