Transcriptomics

Dataset Information

0

Heat shock response in Arabidopsis Hsa32 knockout mutant


ABSTRACT: Plants and animals share similar mechanisms in the heat-shock (HS) response, such as synthesis of the conserved HS proteins (Hsps). However, because plants are confined to a growing environment, in general they require unique features to cope with heat stress. We have analyzed the function of a novel Hsp, heat-stress-associated 32-kD protein (Hsa32), which is highly conserved in land plants but absent in most other organisms. The gene responds to HS at the transcriptional level in moss, Arabidopsis, and rice. Like other Hsps, Hsa32 protein accumulates greatly in Arabidopsis seedlings after HS treatment. Disruption of Hsa32 by T-DNA insertion does not affect growth and development under normal conditions. However, the acquired thermotolerance in the knockout line was compromised following a long recovery period (> 24 h) after an acclimation HS treatment, when a severe HS challenge killed the mutant but not the wild-type plants, but no significant difference was observed if they were challenged within a short recovery period. Microarray analysis of the knockout mutant indicates that only the expression of Hsa32 was significantly altered in HS response. Taken together, our results suggest that Hsa32 is not required for the induction but maintenance of acquired thermotolerance. This report provides direct evidence that a plant-specific Hsp plays an important role in thermotolerance. Keywords: heat shock response

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE4062 | GEO | 2006/03/20

SECONDARY ACCESSION(S): PRJNA95209

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2007-06-27 | E-GEOD-4062 | biostudies-arrayexpress
2008-06-13 | E-GEOD-4760 | biostudies-arrayexpress
2006-11-01 | GSE4760 | GEO
2022-08-08 | PXD035418 | Pride
2005-12-01 | GSE2998 | GEO
2019-05-31 | GSE121182 | GEO
2005-11-30 | E-GEOD-2998 | biostudies-arrayexpress
2014-08-01 | E-GEOD-56611 | biostudies-arrayexpress
2014-08-01 | GSE56611 | GEO
2020-12-23 | PXD021959 | Pride