Granulosa cell differentiation in vivo and developmental competence of oocytes in the bovine model
Ontology highlight
ABSTRACT:
ORGANISM(S): Bos taurus
PROVIDER: GSE40916 | GEO | 2013/06/03
SECONDARY ACCESSION(S): PRJNA175343
REPOSITORIES: GEO
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Project description:Transcriptional profiling of in vivo granulosa cells collected at 20h, 44h, 68h, and 92h of coasting in lactating cow (breed Holstein) 4 conditions experiment, 3 biological replicates, one repliacte per array, dye swap experiment, two colors, 20h vs 44h, 44h vs 68h, 68h vs 92h, 20h vs 68h, 20h vs 92h, 44h vs 92h
2013-06-03 | E-GEOD-40916 | biostudies-arrayexpress
Project description:The objective of the study was to analyze the impact of various lenght FSH withdrawal period (coasting) after the last FSH injection on transcriptome changes in in vivo bovine oocytes at the GV stage. Oocytes were collected from super-stimulated animals after 20, 44, 68 and 92 hours of coasting. For each cow individually (biological replicate), each coasting time was compared to others (i.e. 20 hrs vs 44 hrs; 20 hrs vs 68 hrs; 20 hrs vs 92 hrs; 44 hrs vs 68 hrs; 44 hrs vs 92 hrs and 68 hrs vs 92 hrs) for a total of six comparisons. Overall, 36 hybridizations, corresponding to the three cows and six comparisons were done using a dye-swap set-up.
2013-06-21 | E-GEOD-38345 | biostudies-arrayexpress
Project description:The objective of the study was to analyze the effect of a GnRH antagonist used during the FSH withdrawal period (coasting) on transcriptome changes in in vivo bovine oocytes at the GV stage. Oocytes were collected from super-stimulated animals after a coasting duration of 68hrs (optimal condition, control) and also from the same animal who received the GnRH antagonist (treated) during the whole coasting period. 68hrs of coasting (control) was compared with the 68hrs + GnRH antagonist (treatment), for each cow individually. Overall, 6 hybridizations were done, corresponding to the three biological replicates and one comparison using a dye-swap set-up.
2014-02-25 | E-GEOD-44664 | biostudies-arrayexpress
Project description:The objective of the study was to analyze the impact of various lenght FSH withdrawal period (coasting) after the last FSH injection on transcriptome changes in in vivo bovine oocytes at the GV stage. Oocytes were collected from super-stimulated animals after 20, 44, 68 and 92 hours of coasting.
2013-06-21 | GSE38345 | GEO
Project description:Transcriptional profiling of bovine in-vitro produced blastocysts at day 7 and granulosa cells collected at day 8 to 11 post-eostrus of lactating cows. Two-condition experiment, in-vitro Blastocyst vs. Granulosa cells. Biological replicates: 3 . One replicate per array. Dye swap experiment, two-colors.
2011-07-31 | E-GEOD-27530 | biostudies-arrayexpress
Project description:Transcriptional profiling of bovine in-vitro produced blastocysts at day 7 and granulosa cells collected at day 8 to 11 post-eostrus of lactating cows.
2011-08-01 | GSE27530 | GEO
Project description:Cellular mechanisms that contribute to low estradiol concentrations produced by the preovulatory ovarian follicle in cattle with a compromised metabolic status (such as lactatino) are largely unknown. To gain insight into the main metabolic mechanisms affecting preovulatory follicle function RNAseq profiling was conducted on non-lactating Holstein-Friesian heifers (n=16) and lactating Holstein-Friesian cows (n=17) at three stages of preovulatory follicle development: A) newly selected dominant follicle in the luteal phase (Selection); B) follicular phase before the LH surge (Differentiation) and C) pre-ovulatory phase after the LH surge (Luteinization). Based on a combination of RNA sequencing, ingenuity pathway analysis and Q-RT-PCR validation several important molecular markers involved in steroid biosynthesis, such as the expression of steroidogenic acute regulatory protein (STAR) within developing dominant follicles, were identified to be affected (downregulated) by the catabolic state. We propose that the adverse metabolic environment caused by lactation decreases preovulatory follicle function by affecting cholesterol transport into the mitochondria to initiate steroidogenesis. Granulosa and Theca samples from the dominant follicle were taken from cows and heifers at stages: selection, differentiation and luteinization.
2012-06-15 | E-GEOD-34317 | biostudies-arrayexpress
Project description:Limited oocyte and ovarian reserve in vivo or chemo-therapy leads to reproductive aging or premature aging and associated diseases including infertility. Excitingly, oocytes have been successfully derived from embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) by ectopic expression of transcription factors, showing great potential in fertility preservation or restoration. The accessible granulosa cells are a type of somatic cells that interact and evolve with oocyte development during folliculogenesis. Further, with stem cells-like property, granulosa cells are amenable to reprogramming to generate iPSCs and have been the first used for clone animals. These prompted us to explore the potential of granulosa cells in derivation of germ cells. Meanwhile, the strict genome fidelity required for germ cells inspired us to test reprograming by complete small chemicals, which avoids genetic manipulation, cell transfection and destruction of embryos. Here we show that somatic granulosa cells of adult mouse ovaries can be converted to germ cells and functional oocytes that reproduce fertile pups. We are able to consistently induce granulosa cells to pluripotent state (gPSCs) like ESCs in both developmental competence and molecular signatures. Notably, crotonic sodium-facilitated crotonylation is critical not only for pure small chemicals-based reprogramming of granulosa cells to gPSCs, but also confers the gPSCs with high germline capacity. Consequently, the gPSCs and the derived primordial germ-cell like cells hold longer telomeres and maintain high genomic stability which is critical for germ cells. Taken together, we efficiently generate high quality gPSCs and functional oocytes from adult granulosa cells by significantly improving chemical reprograming approach.
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Project description:Transcriptional profiling of cows granulosa cells comparing granulosa cells from superstimulated control cows with granulosa cells from superstimulated cetrotide treated cows. Abstract:In cow, follicular growth stimulation (FSH) followed by a 44h-68h coasting (no FSH) period has been shown to be beneficial for developmental competence of aspirated oocytes. This process has been optimized but the associated physiology is not perfectly understood. During the above treatment LH pulse frequency is basal as the animal are in the luteal phase. The goal of this study was to test the existence of a beneficial effect of basal LH pulses during competence acquisition. To answer to this question, LH pulses have been pharmacologically inhibited during the optimal coasting period with GnRH antagonists. Commercial milking cycling cows (n=5) were stimulated with 3 days of FSH followed by a coasting period of 68H, with and without cetrorelix injections. At the scheduled time, transvaginal aspirations of immature oocytes were performed, followed by IVF while follicular cells were pooled for each animal and were snap-frozen. Granulosa cells RNA was extracted amplified and hybridized on Embryogene bovine microarray slides. Data were normalized with Loess, and analyzed with Limma procedure. Follicle size group proportions and granulosa cell transcriptome were studied. (1) There was a significant reduction in >10mm diameter follicles with cetrorelix comparing to without treatment. (2) Transcriptome analysis reveals 724 tanscripts potentially regulated by basal gonadotropins before LH surge. Among this LH influenced gene group, 84 transcripts were positively associated to developmental competence. This work allows the identification of subpopulations of gene signals dependant from LH or not, and permits a better comprehension of the implication of basal LH in follicle growth and in competence acquisition process.
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Project description:In this study we sequenced RNA of granulosa collected from Holstein cows at oocyte aspiration prior to IVP, to identify candidate genes and biological mechanisms for favourable IVP-related traits in donor cows.
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