Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Molecular profiling of activated neurons by phosphorylated ribosome capture

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE40995 | GEO | 2012/09/20

SECONDARY ACCESSION(S): PRJNA175508

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Molecular profiling of activated neurons by phosphorylated ribosome capture.

Project description:The mammalian brain is composed of thousands of interacting neural cell types. Systematic approaches to establish the molecular identity of functional populations of neurons would advance our understanding of neural mechanisms controlling behavior. Here, we show that ribosomal protein S6, a structural component of the ribosome, becomes phosphorylated in neurons activated by a wide range of stimuli. We show that these phosphorylated ribosomes can be captured from mouse brain homogenates, thereby enriching directly for the mRNAs expressed in discrete subpopulations of activated cells. We use this approach to identify neurons in the hypothalamus regulated by changes in salt balance or food availability. We show that galanin neurons are activated by fasting and that prodynorphin neurons restrain food intake during scheduled feeding. These studies identify elements of the neural circuit that controls food intake and illustrate how the activity-dependent capture of cell-type-specific transcripts can elucidate the functional organization of a complex tissue.

| S-EPMC3839252 | biostudies-literature

Molecular profiling of activated neurons by phosphorylated ribosome capture

Project description:This SuperSeries is composed of the following subset Series: GSE40969: Molecular profiling of activated neurons by phosphorylated ribosome capture [RNA-Seq] GSE40994: Molecular profiling of activated neurons by phosphorylated ribosome capture [Illumina BeadArray] Refer to individual Series

2012-09-20 | E-GEOD-40995 | biostudies-arrayexpress

Molecular profiling of activated neurons by phosphorylated ribosome capture [Illumina BeadArray]

Project description:We report that phosphorylated ribosomes can be immunoprecipitated from mouse brain homogenates, enriching for mRNAs that were selectively expressed in activated neurons

2012-09-20 | GSE40994 | GEO

Molecular profiling of activated neurons by phosphorylated ribosome capture [RNA-Seq]

Project description:We report that phosphorylated ribosomes can be immunoprecipitated from mouse brain homogenates, resulting in enrichment of transcripts expressed in activated neurons.

2012-09-20 | GSE40969 | GEO

Molecular profiling of activated neurons by phosphorylated ribosome capture [RNA-Seq]

Project description:We report that phosphorylated ribosomes can be immunoprecipitated from mouse brain homogenates, resulting in enrichment of transcripts expressed in activated neurons. Mice were either injected with a concentrated salt solution or vehicle, hypothalami dissected, and phosphorylated ribosomes immunoprecipitated. RNA was sequenced from the input and IP for each condition (4 samples total).

2012-09-20 | E-GEOD-40969 | biostudies-arrayexpress

Molecular profiling of activated neurons by phosphorylated ribosome capture [Illumina BeadArray]

Project description:We report that phosphorylated ribosomes can be immunoprecipitated from mouse brain homogenates, enriching for mRNAs that were selectively expressed in activated neurons Mice were given an injection of concentrated salt solution (2M NaCl, 325 uL) or PBS, and then sacrificed 2h later and hypothalami dissected. Tissue homogenates were prepared from hypothalamus and phosphorylated ribosomes immunoprecipitated using pS6 244/247 antibodies. The immunoprecipitated RNA and total RNA from both control and salt treated animals were then purified and analyzed by Illumina Microarray.

2012-09-20 | E-GEOD-40994 | biostudies-arrayexpress

Molecular diversity of olfactory bulb projection neurons

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-12-05 | GSE162656 | GEO

Suppressed 60S ribosome exportation in maize ribosome export associated 1 mutant triggers inconsistent transcriptional and translational regulations

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-04-25 | E-GEOD-67103 | biostudies-arrayexpress

Ribosome profiling reveals conserved guanosine residues at ribosome pausing sites in Bacillus subtilis

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-01-01 | GSE250314 | GEO