Gene alteration in A549 cells by disinfectant PHMG
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE41156 | GEO | 2012/09/27
SECONDARY ACCESSION(S): PRJNA175938
REPOSITORIES: GEO
Similar Datasets
Project description:Polyhexamethyl guanidine is widely used as a disinfectant in various places such as hospitals and restaurants. In order to examine of PHMG cytotoxicity in lung adenocarcinoma A549 cells we treated 5 ug/ml PHMG for 24 h. To investigate cytotoxicity of commonly used bactericidal reagent PHMG, A549 cells were treated 5 ug/ml PHMG for 24 h. After then purified the total RNA from treated and untreated cells using the Rneasy mini kit (Qiagen). The concentration and purity of the total RNA was measured and performed gene array.
2012-09-27 | E-GEOD-41156 | biostudies-arrayexpress
Project description:Polyhexamethyl guanidine is widely used as a disinfectant in various places such as hospitals and restaurants. In order to examine of PHMG cytotoxicity in lung adenocarcinoma A549 cells we treated 5 ug/ml PHMG for 24 h. To investigate cytotoxicity of commonly used bactericidal reagent PHMG, A549 cells were treated 5 ug/ml PHMG for 24 h. After then purified the total RNA from treated and untreated cells using the Rneasy mini kit (Qiagen). The concentration and purity of the total RNA was measured and performed gene array.
| S-ECPF-GEOD-41156 | biostudies-other
Project description:Inhalation of toxic chemicals, including recent e-cigarettes, often cause life-threatening lung injury. Although exposure to polyhexamethylene guanidine (PHMG)-containing humidifier disinfectant (HD) has been identified as a cause of fatal lung injury, the mechanism underlying HD-associated lung injury (HDLI) is unknown. The present study evaluated global changes in gene expression in lung tissues from patients with PHMG-induced HDLI, and compared gene expression changes in PHMG-induced rat lung tissues. Significantly different expressions in lung tissues between patients with HDLI and unaffected controls were observed. Furthermore, several fibrosis-associated overlapping genes (such as MMP2 and COL1A2) shared between humans with HDLI and rats exposed to PHMG were identified. Interactome network analysis predicted different pathways between children and adults with HDLI: the TGFβ/SMAD signaling pathway was central in adults, whereas other pathways, including integrin signaling, were associated with HDLI in children. Further interactome network analysis revealed that Rap1 and CCKR signaling pathways were significantly enriched in HDLI compared with idiopathic pulmonary fibrosis as well as their recapitulation in the lung tissues of rats exposed to PHMG. Our results suggest that MMP2-mediated different mechanisms between children and adults may be associated with PHMG-induced HDLI development, and Rap1 and CCKR pathways appear to be crucial.
2021-07-06 | GSE179446 | GEO
Project description:Inhalation of toxic chemicals, including recent e-cigarettes, often cause life-threatening lung injury. Although exposure to polyhexamethylene guanidine (PHMG)-containing humidifier disinfectant (HD) has been identified as a cause of fatal lung injury, the mechanism underlying HD-associated lung injury (HDLI) is unknown. The present study evaluated global changes in gene expression in lung tissues from patients with PHMG-induced HDLI, and compared gene expression changes in PHMG-induced rat lung tissues. Significantly different expressions in lung tissues between patients with HDLI and unaffected controls were observed. Furthermore, several fibrosis-associated overlapping genes (such as MMP2 and COL1A2) shared between humans with HDLI and rats exposed to PHMG were identified. Interactome network analysis predicted different pathways between children and adults with HDLI: the TGFβ/SMAD signaling pathway was central in adults, whereas other pathways, including integrin signaling, were associated with HDLI in children. Further interactome network analysis revealed that Rap1 and CCKR signaling pathways were significantly enriched in HDLI compared with idiopathic pulmonary fibrosis as well as their recapitulation in the lung tissues of rats exposed to PHMG. Our results suggest that MMP2-mediated different mechanisms between children and adults may be associated with PHMG-induced HDLI development, and Rap1 and CCKR pathways appear to be crucial.
2021-07-06 | GSE179445 | GEO
Project description:Transcriptional profiling of two disinfectant-resistant Listeria monocytogenes strains indentified in a Iberian pork plant, S1 [160908] vs S10_1 [160908], in presence of 1.25 mg/L of Benzalkonium chloride
2015-01-24 | GSE65232 | GEO
Project description:Transcriptional profiling of two disinfectant-resistant Listeria monocytogenes strains indentified in a Iberian pork plant, S1 [160908] vs S10_1 [160908], in presence of 1.25 mg/L of Benzalkonium chloride One condition experiment, S1 [160908] vs. S10_1 [160908] strains. Biological replicates: 3 replicates of S1 [160908], 3 replicates of S10 [160908].
2015-01-24 | E-GEOD-65232 | biostudies-arrayexpress
Project description:Disinfectants are essential tools for controlling infectious diseases and maintaining sterile conditions in many medical and food-industry settings. Recent work revealed that a deficiency in the PTS carbohydrate phosphor transfer system confers pan-tolerance to killing by diverse disinfectant types through its interaction with the cAMP-CRP regulatory network. The present work characterized a new pan-tolerance mutant obtained by enrichment using phenol as a lethal probe and an Escherichia coli PTS null mutant as a parental strain. The resulting super-pan-tolerant mutant, which harbored an F158C PheS substitution, inhibited bacterial killing by multiple disinfectant classes with surprisingly little effect on antimicrobial lethality. The PheS substitution, which was expected to lower substrate recognition efficiency and result in deacylated tRNAphe occupying the ribosomal A site, activated relA expression and synthesis of ppGpp, even in the absence of disinfectant exposure. ppGpp, along with DksA, increased RpoS function by activating promoters of dsrA and iraP, two genes whose products increase the expression and stability of RpoS. Subsequently, RpoS upregulated the expression of genes encoding a universal stress protein (UspB) and an oxidative stress peroxidase (KatE), which preconditions bacteria to better survive a variety of disinfectants. Disinfectant-mediated accumulation of ROS and bacterial killing were abolished/reduced by an F158C PheS substitution, by exogenous DMSO, and by the PheS F158C substitution up-regulating genes encoding ROS-detoxifying enzymes (katE, sodA, oxyR, ahpC). These data identify a pheS mutation-triggered, ppGpp-stimulated transcriptional regulatory cascade that negates biocide-mediated lethality, thereby tying the stringent response to protection from ROS-mediated biocide lethality.
2024-11-27 | GSE282610 | GEO
Project description:There is emerging interest in using proteomic data for environmental health-risk assessments. Meanwhile, due to attractive physicochemical properties, production and use of engineered nanomaterials (ENMs) are expanding with potential for exposure, thus necessitating toxicity information on these materials for human health risk analysis, where proteomic data can be informative. Here, cells (A549 human lung epithelial, J774 mouse monocyte/macrophage) were exposed to ENMs (nanoforms of SiO2, TiO2) of different sizes, surface chemistries (dose:0-100 ug/cm2, 24 h) for in vitro toxicity data. Cellular cytotoxicity (CTB, ATP, LDH), oxidative stress and proteomic analysis (MS-, antibody-based) were conducted post-nanoparticle (NP) exposures to determine cytotoxic potencies and identify molecular-level effects. Dose-, nanoform-, cell type-specific cytotoxicity changes were seen with both nanoSiO2 and nanoTiO2 exposures. Size, agglomeration, surface groups and metal impurities appeared to be cytotoxicity determinants. Proteomic data identified some common enriched mechanistic pathways relevant to phagocytosis, cell-shape changes, apoptosis, and inflammatory processes for nanoSiO2, nanoTiO2 exposed J774 cells. A549 cells exhibited pathway changes relevant to cell metabolism, endocytosis and inflammatory processes post-NP exposures. Concordance was seen between, cytotoxicity responses, notably cellular ATP, critical for cell viability, cellular oxidative stress and affected cellular pathways. These findings demonstrate the utility of proteomics in toxicology, warranting further exploration.
2024-08-12 | MSV000095579 | MassIVE
Project description:Antimicrobial chemicals are widely applied to clean and disinfect food-contacting surfaces. However, the cellular response of bacteria, such as Bacillus cereus, to various disinfectants is unclear. In this study, the physiological and genome-wide transcriptional responses of B. cereus ATCC 14579 exposed to four different disinfectants (i.e., benzalkonium chloride, sodium hypochlorite, hydrogen peroxide, and peracetic acid) were analyzed. The physiological response of B. cereus to different concentrations of the disinfectants used was investigated. For each disinfectant, concentrations leading to the attenuation of growth, growth arrest, and cell death were studied in more detail. The simultaneous analysis of the transcriptional responses of B. cereus upon exposure to the different concentrations of disinfectants revealed common responses induced by the four disinfectants. Notably, genes involved in the general and oxidative stress responses were commonly up-regulated. Furthermore, the obtained results indicate that all the disinfectants also induce specific responses. Exposure to benzalkonium chloride, a disinfectant known to induce membrane damage, specifically induced genes involved in the fatty acid metabolism. Benzalkonium chloride induced-membrane damage was confirmed by fluorescence microscopy and fatty acid analysis confirmed that fatty acid composition of cell membrane was affected upon exposure to benzalkonium chloride. Sodium hypochlorite induced genes involved in sulfur and sulfur-containing amino acids metabolism, which correlated with the observed sodium hypochlorite-specific induction of oxidation of sulphydryl groups. Hydrogen peroxide and peracetic acid exposures induced genes involved in DNA damage and the SOS response. Notably, hydrogen peroxide and peracetic acid-treated cells exhibited higher mutation rates corroborating with the induced SOS response. Understanding the mechanisms displayed by microorganisms coping with disinfectants-induced stress may allow for design of more efficient sequential and/or disinfectant combination treatments in food processing environments.
2010-03-31 | GSE18807 | GEO
