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Gene expression profiling of a nisin-sensitive Listeria monocytogenes Scott A ctsR deletion mutant

ABSTRACT: Transcriptional profling of a Listeria monocytogenes under nisin treatment comparing ctsR mutant and wild type

ORGANISM(S): Listeria monocytogenes str. Scott A Listeria monocytogenes

PROVIDER: GSE41891 | GEO | 2013/02/14

SECONDARY ACCESSION(S): PRJNA178403

REPOSITORIES: GEO

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Gene expression profiling of a nisin-sensitive Listeria monocytogenes Scott A ctsR deletion mutant

Project description:Transcriptional profling of a Listeria monocytogenes under nisin treatment comparing ctsR mutant and wild type one condition (nisin treament 20ug/ml, 24 hours) experiment, ctsR mutant vs. wild type Listeria monocytogenes Scott A, 2 biological replicates, 4 technical replicates

2013-02-14 | E-GEOD-41891 | biostudies-arrayexpress

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Project description:transcriptional profiling of L. monocytogenes ctsR mutant under pressure treatment SUBMITTER_CITATION: Liu, Y., Huang, L., Joerger, R.D., Gunther, N.W. 2012. Genes that are involved in high hydrostatic pressure treatments in a Listeria monocytogenes Scott A ctsR deletion mutant. Journal of Microbial and Biochemical Technology. 4:050-056.

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Listeria monocytogenes exposure to chlorine dioxide

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Project description:Listeria monocytogenes strain 10403S has been studied extensively for stress response activity toward multiple stressors (acid, osmotic, cold, high temperature, etc.) as well as multiple stress regulons (SigB, CtsR, HrcA, etc.). Here we aimed to determine the transcriptional response of Listeria monocytogenes in early log phase towards the strong oxidative stress imposed by ClO2. The elucidation of such a response allows for further a more completel understanding of the mechanism of inactivation by sanitizers, specifically ClO2. Independent RNA isolations were performed for strain 10403S with and without exposure to ClO2 from cells grown to early log phase. Four biological replicates were used in competitive whole-genome microarray experiments. For each set of hybridizations, RNA from a control sample of Listeria monocytogenes was hybridized with RNA from a culture of L. monocytogenes following exposure to ClO2. Dye swapping was performed for the four replicates to mitigate any concerns of dye bias.

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