Changes in Expression of Genes Involved in Apoptosis in Activated Human T-Cells in Response to Modeled Microgravity
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ABSTRACT:
ORGANISM(S): Homo sapiens
PROVIDER: GSE4209 | GEO | 2006/02/09
SECONDARY ACCESSION(S): PRJNA95067
REPOSITORIES: GEO
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Project description:The purpose of this study was to search for microgravity-sensitive genes, specifically for apoptotic genes influenced by the microgravity environment and other genes related to immune response. Experiment Overall Design: Two-group design with paired samples, i.e., one 1G and one MMG culture came from the same donor. Therefore, 6 samples came from 3 different donors. Experiment Overall Design: Donor 1 :GSM96146,GSM96147 Experiment Overall Design: Donor 2: GSM96148, GSM96149 Experiment Overall Design: Donor 3: GSM96150,GSM96151 Experiment Overall Design: Total RNA was submitted to, and then labeled, hybridized and data generated by the Baylor College Medicine Microarray Core Facility (333E One Baylor Plaza, Houston, TX 77030).
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Project description:The goal of this study was to assess whether low shear-modeled microgravity (LSMMG) effects yeast ,genomic expression patterns using the powerful tool of whole genome microarray hybridization. We determined ,changes in the yeast model organism, Saccharomyces cerevisisae, when grown in LSMMG using the rotating High ,Aspect Ratio Vessel (HARV). A significant number of genes were up- or down-regulated by at least two fold in cells ,that were grown for 5 generations or 25 generations in HARVs. We identified genes in cell wall integrity signaling ,pathways containing MAP kinase cascades that may provide clues to novel physiological responses of eukaryotic ,cells to the external stress of a low-shear modeled microgravity environment. A comparison of the microgravity ,response to other environmental stress response (ESR) genes showed that 26% of the genes that respond ,significantly to LSMMG are involved in a general environmental stress response, while 74% of the genes may ,represent a unique transcriptional response to microgravity. In addition, we found changes in genes involved in ,budding, cell polarity establishment, and cell separation that confirm our hypothesis that exposure to LSMMG ,causes changes in gene transcription resulting in a phenotypic response. The results of the study provide interesting ,clues to potential mechanisms involved in the response to, adaptation to, and survival of eukaryotic cells in a ,microgravity environment and our findings may have important health implications for human spaceflight. Experiment Overall Design: Four conditions are compared with three replicates each: yeast grown in low-shear modeled microgravity (HARV bioreactor) for 5 and 25 generations; yeast grown in a horizontal (non-LSMMG) HARV bioreactor for 5 and 25 generations.
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