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Ly6Chi monocytes in the inflamed colon give rise to pro-inflammatory effector cells and migratory antigen presenting cells.

ABSTRACT: We showed different function of monocyte derived cells in the lamina propria of the colon under steady state and inflammatory conditions. We used microarrays to detail the global programme of gene expression and identified distinct clusters of regulated genes during this process.

ORGANISM(S): Mus musculus

PROVIDER: GSE42101 | GEO | 2012/11/08

SECONDARY ACCESSION(S): PRJNA179136

REPOSITORIES: GEO

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Ly6Chi monocytes in the inflamed colon give rise to pro-inflammatory effector cells and migratory antigen presenting cells.

Project description:We showed different function of monocyte derived cells in the lamina propria of the colon under steady state and inflammatory conditions. We used microarrays to detail the global programme of gene expression and identified distinct clusters of regulated genes during this process. Different subsets of mononuclear phagocytes were sorted from the colonic lamina propria as well as the spleen. Sorting was done in C57BL/6 mice in steady state and under inflammatory conditions (Dextran Sodium Sulphate induced colitis model)

2012-11-08 | E-GEOD-42101 | biostudies-arrayexpress

Transcriptional profiles of intestinal antigen presenting cells from control, HFD-induced prediabetic and diabetic mice

Project description:To determine whether diet-induced changes in gut microbiota modified intestinal immune cell gene expression, we analyzed the transcriptome of antigen presenting cells isolated from the lamina propria of the small intestine of mice fed with different diet.

2015-11-18 | GSE52557 | GEO

Genome-wide analysis of tissue-specific gene expression by Ly6Chi inflammatory monocytes during Toxoplasma gondii infection

Project description:Analysis of Ly6Chi monocytes from small intestine lamina propria (SILP) and blood of day 8 Toxoplasma gondii infected mice at gene expression level. The hypothesis tested in the present study was that Ly6Chi monocytes from SILP have altered expression of regulatory factors to blood monocytes. Results provide important information on the regulatory to effector balance of genes expressed by Ly6Chi monocytes during an acute inflammatory response. Ly6Chi inflammatory monocytes were sorted by FACS from the blood or small intestine lamina propria (SILP) of Toxoplasma gondii infected C57BL/6 mice. Cells were isolated at day 8 after infection and total RNA obtained from sorted populations. Three biological replicates were acquired for both blood and SILP from pooled animals.

2013-05-25 | E-GEOD-46685 | biostudies-arrayexpress

next generation sequencing of migratory Innate lymphoid cells, T cells and dendritic cells in mesenteric lymph

Project description:This study was designed to identify NGS-derived trascriptomes (RNA-sequencing) of different cell populations (ILCs, T cells and DCs) isolated from the lamina propria of the intestine and from the thoracic duct.

2020-10-27 | GSE160156 | GEO

Genome-wide analysis of tissue-specific gene expression by Ly6Chi inflammatory monocytes during Toxoplasma gondii infection

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Next-generation sequencing of migratory innate lymphoid cells, T cells and dendritic cells in mesenteric lymph [CD11a KO DC]

Project description:This study was designed to identify NGS-derived transcriptomes (RNA-sequencing) of different cell populations (ILCs, T cells and DCs) isolated from the lamina propria of the intestine and from the thoracic duct. For the DC subset, CD11a WT and KO samples were used.

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Stem-like intestinal Th17 cells give rise to pathogenic effector T cells during autoimmunity.

Project description:While intestinal Th17 cells are critical for maintaining tissue homeostasis, recent studies have implicated their roles in the development of extra-intestinal autoimmune diseases including multiple sclerosis. However, the mechanisms by which tissue Th17 cells mediate these dichotomous functions remain unknown. Here, we characterized the heterogeneity, plasticity, and migratory phenotypes of tissue Th17 cells in vivo by combined fate mapping with profiling of the transcriptomes and TCR clonotypes of over 84,000 Th17 cells at homeostasis and during CNS autoimmune inflammation. Inter- and intra-organ single-cell analyses revealed a homeostatic, stem-like TCF1+ IL-17+ SLAMF6+ population that traffics to the intestine where it is maintained by the microbiota, providing a ready reservoir for the IL-23-driven generation of encephalitogenic GM-CSF+ IFN-γ+ CXCR6+ T cells. Our study defines a direct in vivo relationship between IL-17+ non-pathogenic and GM-CSF+ and IFN-γ+ pathogenic Th17 populations and provides a mechanism by which homeostatic intestinal Th17 cells direct extra-intestinal autoimmune disease.

| S-EPMC8900676 | biostudies-literature

Development of gene expression signatures for colonic lamina propria DCs of CD300a deficient mice

Project description:To further development of our gene expression approach to CD300a deficiency on dendritic cells (DCs) in colonic lamina propria, we have employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to distinguish CD300a deficiency on DCs in colonic lamina propria from those of WT mice. Colonic lamina propria DCs were obtained by cell sorter from WT and CD300a deficient mice raised under SPF and GF condition. Expression of Ifnb1 was significantly higher in CD300a deficient DCs, quantified in the same RNA samples by real-time PCR. Gene expression in WT and CD300a colonic lamina propria DCs raised under SPF and GF conditions were measured. Colonic lamina propria cells were obtained from 5 mice in each conditions. Takara-Bio

2015-04-10 | E-GEOD-67696 | biostudies-arrayexpress

Transcriptional profile of Foxp3+ regulatory T cells from colon lamina propria and mesenteric lymph node in steady state mice

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An IL-23R/IL-22 circuit regulates epithelial serum amyloid A to promote local effector Th17 responses [RNA-Seq]

Project description:Purpose: The goals of this study are to compare mRNAs expressed by Th17 cells and ILC3s in small intestine of lamina propria of mice. Methods: Small intestine were digested with collagenase, dispase, and DNase. Percoll enriched lamina propria Th17 and ILCs sorted by BD ARIA II. Total RNA were harvested and sequencing were performe. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) and TopHat followed by CuffDiff. qRT–PCR validation was performed using SYBR Green assays. Results: Small intestine Th17 cells and ILCs exhibit differential gene expression profiles.

2015-10-09 | GSE70596 | GEO

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