Transcriptomics

Dataset Information

0

CFIm25 links alternative polyadenylation to glioblastoma tumour suppression


ABSTRACT: Purpose: To identify all of the APA targets of CFIm25 on a global scale and develop an algorithm that can idenitify APA events from standard RNA-seq data Methods: RNA from HeLa cells treated with control siRNA and CFIm25 siRNA were subject to RNA-Seq. Using a custom-designed algorithm to mine RNA-seq data for novel APA events regulated by CFIm25. Results: We identified over 1,400 genes with shortened 3’UTRs after CFIm25 knockdown. Importantly, we show that as a consequence of APA, many of these mRNAs have greatly enhanced protein expression due to the loss of destabilizing features within the 3’UTR. Conclusions: Our study underscored the critical function of the CFIm complex members in governing APA and establish a previously unknown link between APA and metabolic pathways important for tumor progression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE42420 | GEO | 2014/05/15

SECONDARY ACCESSION(S): PRJNA182153

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2014-05-15 | E-GEOD-42420 | biostudies-arrayexpress
2018-02-20 | GSE78198 | GEO
2022-02-11 | PXD027034 | Pride
2022-02-16 | GSE179630 | GEO
2019-08-14 | PXD014842 | Pride
2020-04-27 | GSE135384 | GEO
2021-09-10 | GSE183704 | GEO
2020-04-15 | GSE142681 | GEO
2020-04-15 | GSE142682 | GEO
2021-05-04 | GSE158591 | GEO