Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of synbindin on the gene expression profile of gastric cancer cells

ABSTRACT: In order to determine the effect of synbindin on the gene expression profile of gastric cancer cells, MGC-803 cells were infected with adenoviruses carrying either synbindin or GFP. The differentially expressed genes caused by synbindin ectopic expression suggests the underlying mechanism for synbindin-induced cell proliferation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE42736 | GEO | 2012/12/05

SECONDARY ACCESSION(S): PRJNA182966

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Effect of synbindin on the gene expression profile of gastric cancer cells

Project description:In order to determine the effect of synbindin on the gene expression profile of gastric cancer cells, MGC-803 cells were infected with adenoviruses carrying either synbindin or GFP. The differentially expressed genes caused by synbindin ectopic expression suggests the underlying mechanism for synbindin-induced cell proliferation. Total RNA from each sample was amplified and transcribed into fluorescent cRNA using Agilent's Quick Amp Labeling protocol (version 5.7, Agilent Technologies). The labeled cRNAs were hybridized onto the Whole Human Genome Oligo Microarray (4 x 44K, Agilent Technologies). After washing, the arrays were scanned by the Agilent Scanner G2505C. 3 replicates per treatment (GFP adenovirus, synbindin adenovirus).

2012-12-05 | E-GEOD-42736 | biostudies-arrayexpress

Overexpression of TREM2 in human gastric cancer cells

Project description:In order to investigate the molecular mechanisms underlying the role of TREM2 in gastric cancer, deep sequencing to profile global gene expression of MGC-803 cells with TREM2 overexpression was performed.

2017-11-01 | GSE106387 | GEO

Transcriptome Analysis of CircMRPS35 mediated mRNA expression profiles

Project description:Purpose: To explore the mechanism of circMRPS35-mediated suppression of proliferation and invasion in gastric cancer. Methods: MGC-803 cells were transfected with control and SiRNA-circMRPS35, in triplicate. Total RNA was extracted using Trizol reagent.

2019-11-01 | GSE121382 | GEO

Essential Oil from Pinus Koraiensis pinecones Inhibits Gastric Cancer Cells via the HIPPO/YAP Signaling Pathway

Project description:The purpose of this study was to investigate the anti-tumor activity of PEO on MGC-803 cells and its mechanism. Anti-tumor experiments in vitro showed PEO could significantly inhibit the proliferation and migration of MGC-803 cells, and it also could arrest the cell cycle in G2/M phase, decrease the mitochondrial membrane potential and induce apoptosis. Finally, the effects of PEO on genes expression on MGC-803 cells were analyzed by RNA sequencing, and results showed that after treatment with PEO, 100 genes were up-regulated, and 57 genes were down-regulated. According to KEGG pathway and GSEA, FAT4, STK3, LATS2, YAP1 and AJUBA were down-regulated, which are related to HIPPO signaling pathway. Real-time PCR and western blot further confirmed the results of RNA sequencing. These results indicated that PEO may exert anti-tumor activity via the HIPPO/YAP signaling pathway.

2020-04-14 | GSE137480 | GEO

Construction of a Competing Endogenous RNA Network and Identification of Potential Regulatory Axes in Gastric Cancer Chemoresistance

Project description:Purpose: Emerging evidence highlights the multifunctional role of noncoding RNAs (ncRNAs) in gastric cancer (GC) chemoresistance. However, the comprehensive expression profile and competing endogenous RNAs (ceRNAs) regulatory network of GC chemoresistance remain unanswered. Methods: The whole-transcriptome sequencing (RNA sequencing) was performed to comprehensively analyze the differentially expressed (DE) lncRNAs, miRNAs and mRNAs in cisplatin-resistant cells MGC-803/DDP and GC cells MGC-803. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted to investigate the biological functions implicated with the DEncRNAs. Then, the cisplatin-resistant-related ceRNA network and potential regulatory axes were constructed by bioinformatic analysis. Results: We successfully generated cisplatin-resistant GC cell line MGC-803/DDP. Differential expression analysis showed that a total of 1,936 DElncRNAs, 2,194 DEmRNAs and 174 DEmiRNAs were identified. Functional enrichment analysis indicated that those DEncRNAs were mainly involved in neuroactive ligand-receptor interaction, drug metabolism and Hippo signaling pathway. Subsequently, the cisplatin-resistant-related ceRNA network was constructed with the widely accepted vital chemo-resistant-related genes and signaling pathways. In addition, two constructed regulatory axes (include FAM66C/miR-129-5p/7 mRNAs and SFTA1P/miR-206/FN1 or NRP1) were successfully validated by the Genomic Data Commons (GDC) GC data. Conclusion: The novel ceRNA network and the potential regulatory axes may provide the most comprehensive view of GC chemoresistance to date. Our findings uncovered potential biomarkers for prognostic prediction and novel therapeutic targets for reversing cisplatin resistance in GC.

2021-12-29 | GSE192631 | GEO

Screening target genes of miR-100 in MGC-803 and SK-BR-3 cells

Project description:We tried to find the target genes of miR-100 in MGC-803 and SK-BR-3 cells, thus we uesd specific miR-100 inhibitor to knock down the level of miR-100 in the cells, with this condition, we used this mRNA microarray to find the genes whose amount changed. and they may be the target genes that miR-100 mediated. Total of four chips, MGC-AMO, MGC-NC, SK-AMO, SK-NC. MGC and SK represents MGC-803 and SK-BR-3 cells respectively. AMO is the specific miR-100 inhibitor and NC is negative control.

2014-01-09 | E-GEOD-53909 | biostudies-arrayexpress

Parthenolide induces ROS-dependent cell death in human gastric cancer cells

Project description:Parthenolide (PN), a key active ingredient of feverfew, has been used to treat gastrointestinal disorders. However, the mechanism of the cytotoxic effect exerted by PN on tumor cells is not elucidated now. MGC-803 cells treated with and without PN were separately subjected to high throughput RNA sequencing (RNA-Seq).

2023-12-31 | GSE217526 | GEO

Comprehensive analysis of the immune implication of NOTCH1 gene in stomach adenocarcinoma

Project description:In order to better understand the relationship between NOTCH1 gene and immunity in gastric adenocarcinoma cells, we performed the silent interference of NOTCH1 gene on three gastric adenocarcinoma cell lines AGS, MKN-45 and MGC-803. We extracted the total RNA from the interfered and untreated blank control cells, and performed lncRNA library construction and sequencing. The results of bioinformatics analysis showed that the silencing of NOTCH1 gene in gastric adenocarcinoma cells activated several immune-related signal transduction pathways, including T cell receptors, chemokines, JAK-STAT, Toll-like receptors, ECM receptors and other signals. Conduction pathway. These results confirm that NOTCH1 may down-regulate the immune response of gastric adenocarcinoma.

2023-05-01 | GSE175445 | GEO

Characterization of MGC-803 cell line use sample HeLa and AGS

Project description:Investigation of the mixed origins of the MGC-803 cell line reveals it is a HeLa hybrid cell line

2023-07-11 | GSE236670 | GEO

Characterization of mixed origins MGC 803 cell line

Project description:Investigation of the mixed origins of the MGC 803 cell line reveals it is a nasopharyngeal HeLa hybrid cell line

2023-04-03 | GSE228476 | GEO