Transcriptomics

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Gene expression profiling after Wig-1 knockdown in HCT116 cancer cell line


ABSTRACT: Wig-1 is a p53 target gene that encodes an RNA-binding protein involved in regulation of mRNA stability through binding to AU-rich elements (AREs). The aims of this study were to identify novel Wig-1 target mRNAs and to characterize the mechanisms of their regulation. Using a microarray approach, we identified 2447 transcripts with >4-fold differential expression between Wig-1 siRNA and control siRNA treated HCT116 cells. Among the deregulated transcripts we found a number of p53 target genes. We demonstrated that Wig-1increases 14-3-3σ transcription while it binds to an ARE in the FAS 3’UTR and decreases the FAS mRNA stability. Furthermore, we show that Wig-1 depletion favours cell death rather that cell cycle arrest after DNA damage. We propose a role of Wig-1 in directing the p53 stress response towards cell cycle arrest rather than cell death through regulation of 14-3-3σ and FAS mRNA levels.

ORGANISM(S): Homo sapiens

PROVIDER: GSE43046 | GEO | 2014/03/01

SECONDARY ACCESSION(S): PRJNA184694

REPOSITORIES: GEO

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