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Senescence impairs direct conversion of human somatic cells to neurons

ABSTRACT: Direct conversion from fibroblasts to neurons is a potential cell replacement therapy for neurological disorders, and a variety of combinations of transcription factors have been tried. We notice that the efficiency of conversion from aging fibroblasts was much lower than in early stage cells, which is consistent with the notion that cellular senescence impairs conversion of fibroblasts to neurons. Here, we found that the transient knockdown of the p16Ink4a/p19Arf locus was sufficient to convert human fibroblasts to neurons. Futhermore, expression of hTERT alone, another mechanism behind immortalization, also induced neuron conversion. Our results show that the acquisition of immortality is a crucial step for the conversion of human fibroblasts into induced neurons.

ORGANISM(S): Homo sapiens

PROVIDER: GSE43175 | GEO | 2012/12/28

SECONDARY ACCESSION(S): PRJNA184842

REPOSITORIES: GEO

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