Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HeLa E2F1 ChIP on 24,000 Promoters

ABSTRACT: 2 biological replicates (array 59066 and array 64695) of HeLa E2F1 ChIP vs matched Total DNA Keywords: ChIP

ORGANISM(S): Homo sapiens

PROVIDER: GSE4319 | GEO | 2006/03/03

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

HeLa E2F1 ChIP-chip experiments

Project description:These are three biological replicates. HeLa cells were crosslinked on separate days. In each case, a matched total from the same crosslinking was produced in parallel. Amplicons were made from each and hybridized onto ENCODE region tiling arrays. Two color arrays were used; ChIP was red, Total was green. Goal was determination of E2F1 binding sites in HeLa cells by using ChIP-chip methodology. See Farnham Lab website for downloadable protocol for ChIP or supplementary file (Chip_protocol.txt) below. NimbleGen performed all array processing. See NimbleGen website for information on array procedures.

2010-07-01 | E-GEOD-4306 | biostudies-arrayexpress

HeLa E2F1 ChIP-chip experiments

2006-02-28 | GSE4306 | GEO

IgG control ChIP in HeLa cells

Project description:Control IgG ChIP vs matched total for HeLa cells. Three biological replicates. Keywords: ChIP-chip

2006-03-09 | GSE4338 | GEO

IgG control ChIP in HeLa cells

Project description:Control IgG ChIP vs matched total for HeLa cells. Three biological replicates. ChIP-chip of IgG; look for non-specific binding sites.

2010-07-01 | E-GEOD-4338 | biostudies-arrayexpress

ENCODE ChIP-chip for STAT1 on Human Hela S3 Cells

Project description:STAT1 ChIP-chip performed on Human Hela S3 Cells for three different platforms. Nimblegen ENCODE arrays which comprise 50mer oligonucleotides spaces every 38bps (overlapping by 12nts) (5 biological replicates), custom maskless array tiling most of ENCODE with 50mer oligonucleotides end-to-end (3 biological replicates) and custom maskless array tiling most of ENCODE with 36mer oligonucleotides end-to-end (2 biological replicates). The chromatin-immunoprecipitation protocol is the same for all samples, however the labelling and hybridization protocols differ between Nimblegen and custom maskless arrays. Keywords = Transcription Factor Binding, STAT1, ChIP-chip, Human, Genome Tiling Arrays Keywords: other

2005-05-27 | GSE2714 | GEO

miRNA expression analysis of E2F1 knockdown cells compared to control siRNA treated cells

Project description:To have a global picture of the miRNAs dependent on the transcription factor E2F1, we assessed small RNA changes, by RNA-sequencing, of HeLa cells transfected with an siRNA targeting E2F1 or a control siRNA.

2021-04-12 | GSE147361 | GEO

E2F1 ChIP-Seq on mouse hepatocytes overexpressing E2F1

Project description:E2F transcription factors are known regulators of the cell cycle, proliferation, apoptosis and differentiation. We reveal an essential role for E2F1 in liver through the regulation of glycolysis and lipogenesis. E2F1 deficiency leads to a decreased in glycolysis and de novo synthesis of fatty acids in hepatocytes. ChIP-Seq was performed to determine direct tagets of E2F1 in hepatocytes. We highlight that E2F1 directly binds the promoters of genes implicated in metabolic process and notably key lipogenic genes to control these pathways.

2015-12-01 | GSE74006 | GEO

Profiling gene expression in HeLa cells by hsa-mir-26b overexpression

Project description:Total RNA samples from three biological replicates in which the hsa-mir-26b was overexpressed in HeLa cells were profiled by gene expression. As negative control, we used total RNA samples from HeLa cells transfected with cel-mir-67 Keywords: gene expression array-based (RNA / in situ oligonucleotide)

2008-12-17 | GSE12091 | GEO

Profiling gene expression in HeLa cells by hsa-mir-98 overexpression

Project description:Total RNA samples from three biological replicates in which the hsa-mir-98 was overexpressed in HeLa cells were profiled by gene expression. As negative control, we used total RNA samples from HeLa cells transfected with cel-mir-67 Keywords: gene expression array-based (RNA / in situ oligonucleotide)

2008-12-17 | GSE12092 | GEO

E2F1 binding sites in MCF7 cells ENCODE

Project description:E2F1 binding sites in MCF7 cells were determined in the ENCODE regions Keywords: ChIP-chip

2006-03-09 | GSE4355 | GEO