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Gene expression analysis of B cell differentiation using B cell blasts and developmentally arrested plasmablasts

ABSTRACT: In vitro generated blasts from B cells deficient in prdm1 were isolated according to whether they were attempting to express Blimp1, reported by a GFP cassette knocked into the prdm1 locus

ORGANISM(S): Mus musculus

PROVIDER: GSE43213 | GEO | 2013/02/03

SECONDARY ACCESSION(S): PRJNA184935

REPOSITORIES: GEO

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Gene expression analysis of B cell differentiation using B cell blasts and developmentally arrested plasmablasts

Project description:In vitro generated blasts from B cells deficient in prdm1 were isolated according to whether they were attempting to express Blimp1, reported by a GFP cassette knocked into the prdm1 locus After four days stimulation with CD40L, IL4 and IL5, cells were FACS sorted into GFP+ and GFP- populations, RNA extracted and gene expression analysed by microarray. Data presented in Peparzak et al, 2013, Fig 3b.

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