Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sex Differences in Response to Plasmodium chabaudi Infection: Involvement of Gonadal Steroids

ABSTRACT: The goal of this study was to examine whether immune responses to Plasmodium chabaudi infection differ between the sexes and are altered by the presence of gonadal steroids. Gonadally-intact males were more likely than intact females to die following P. chabaudi infection, exhibit slower recovery from infection-associated weight loss, hypothermia, and anemia, have reduced IFNγ-associated gene expression and IFNγ production during peak parasitemia, and produce less antibody during the recovery phase of infection. Gonadectomy of male and female mice altered these sex-associated differences, suggesting that sex steroid hormone, in particular androgens and estrogens, may modulate immune responses to infection. Keywords: Time Course

ORGANISM(S): Mus musculus

PROVIDER: GSE4324 | GEO | 2006/06/01

SECONDARY ACCESSION(S): PRJNA94747

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of mouse sex differences in response to Plasmodium chabaudi infection: involvement of gonadal steroids

Project description:The goal of this study was to examine whether immune responses to Plasmodium chabaudi infection differ between the sexes and are altered by the presence of gonadal steroids. Gonadally-intact males were more likely than intact females to die following P. chabaudi infection, exhibit slower recovery from infection-associated weight loss, hypothermia, and anemia, have reduced IFNÎ³-associated gene expression and IFNÎ³ production during peak parasitemia, and produce less antibody during the recovery phase of infection. Gonadectomy of male and female mice altered these sex-associated differences, suggesting that sex steroid hormone, in particular androgens and estrogens, may modulate immune responses to infection. Experiment Overall Design: Intact and gonadectomized (gdx) male and female C57BL/6 mice were inoculated with 106 P. chabaudi AS-infected erythrocytes and responses to infection were monitored. For microarray analyses, RNA was isolated from 5 x 106 white blood cells (WBCs) that were isolated from spleens 0, 3, 7, or 14 days after inoculation with P. chabaudi. Equivalent aliquots of RNA from 3 animals/treatment group were pooled and 3 separate pools were processed on separate Affymetrix GeneChips (n = 3 GeneChips/time point/treatment group).

2008-11-22 | E-GEOD-4324 | biostudies-arrayexpress

Plasmodium chabaudi

Project description:WGS of P. chabaudi chabaudi AS clone

| PRJEB2037 | ENA

Whole spleen transcriptome during acute phase of infection in a virulent Plasmodium chabaudi chabaudi CB infection

Project description:Analysis of spleen samples taken throughout the acute phase of infection from mice infected with virulent P. chabaudi CB strain spleen samples collected at 2-day intervals till parasite load were controlled

2020-06-03 | GSE145781 | GEO

Epigenetic modifications of gene promoters in the liver of Balb/c mice protected by vaccination against blood-stage malaria of Plasmodium chabaudi

Project description:Protective vaccination induces survival of more than 80% of female Balb/c mice otherwise succumbing to blood-stage malaria of Plasmodium chabaudi. This study investigates the effect of protective vaccination and P. chabaudi infections on DNA methylation of gene promoters in the liver of Balb/c mice. Using DNA methylation microarrays, protective vaccination is shown to affect per se the constitutive DNA methylation status of numerous gene promoters in the liver: Promoters of 256 genes are hyper(=up)- and 345 genes are hypo (=down)-methylated at a significance of p<0.05. Gene enrichment analysis reveals that genes with down-methylated promoters are most statistically significant enriched (p<0.01) with functions related to negative regulation of transcription, whereas genes with up-methylated promoters are related to positive regulation of transcription and immune system. Infections with P. chabaudi also induce changes in promoter DNA methylation. In vaccinated mice, these changes are observable already on day 1 p.i. and, in particular, at peak parasitemia on day 8 p.i., when 571 and 1013 gene promoters have become up- and down-methylated, respectively, in relation to constitutive DNA methylation at a signicance level of p<0.05. The corresponding gene promoters in non-vaccinated mice are also responsive to infection, but by far not as pronounced as in vaccinated mice. Our data demonstrate that vaccination modifies DNA methylation of gene promoters in the liver and augments promoter responsiveness to infection-induced DNA methylation alterations, which ultimately control expression of those genes in the liver required for survival of otherwise lethal blood-stage malaria of P. chabaudi.

2019-09-23 | GSE87373 | GEO

Plasmodium chabaudi chabaudi

Project description:Characterization and gene expression analysis of the cir multi-gene family of Plasmodium chabaudi chabaudi (AS)

| PRJNA148959 | ENA

Plasmodium chabaudi chabaudi

Project description:Plasmodium chabaudi whole genome shotgun sequencing project

| PRJNA147 | ENA

Whole blood transcriptome during acute phase of infecion in avirulent and virulent Plasmodium chabaudi chabaudi infection

Project description:Analysis of blood samples taken throughout the acute phase of infection from mice infected with avirulent P. chabaudi AS strain or virulent CB strain The influence of parasite genetic factors on immune responses and development of severe pathology of malaria is largely unknown. In this study, we performed genome-wide transcriptomic profiling of whole blood during blood-stage infections of two strains of the rodent malaria parasite Plasmodium chabaudi that differ in virulence. We identified several transcriptomic signatures associated with the virulent infection, including signatures for lung inflammation, stronger and prolonged anemia and platelet aggregation. The latter two signatures were detected prior to pathology. The anemia signature indicated deregulation of host erythropoiesis, and the lung inflammation signature was linked to increased neutrophil infiltration, more cell death and greater parasite sequestration in the lungs. This comparative whole-blood transcriptomics profiling of virulent and avirulent malaria infections shows the validity of this approach to inform severity of malarial infection and provide insight into pathogenic mechanisms.

2017-02-01 | GSE93631 | GEO

Complete DNA sequence of Plasmodium chabaudi chabaudi

Project description:Analysis of the complete DNA sequence of Plasmodium chabaudi chabaudi

| PRJEB1164 | ENA

Whole spleen transcriptome during acute phase of infection in an avirulent Plasmodium chabaudi chabaudi AS infection.

Project description:Whole spleen transcriptome during acute phase of infection in an avirulent Plasmodium chabaudi chabaudi AS infection.

| PRJNA508619 | ENA

Whole spleen transcriptome during acute phase of infection in a virulent Plasmodium chabaudi chabaudi CB infection

Project description:Whole spleen transcriptome during acute phase of infection in a virulent Plasmodium chabaudi chabaudi CB infection

| PRJNA608201 | ENA