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Heterokaryon RNA Sequencing

ABSTRACT: We report the application of bi-species RNAseq for investigating mechanisms of reprogramming towards pluripotency using heterokaryons (mouse embryonic stem cell X human fibroblast cell fusions). The use of mixed species allows one to monitor reprogramming of the human somatic nuclei independently of contributions from the mouse nuclei using nucleotide differences. We used RNAseq to monitor heterokaryon reprogramming over a 3-day timecourse, generating transcriptome-wide data for cell fusion based reprogramming of human fibroblasts towards pluripotency.

OTHER RELATED OMICS DATASETS IN: PRJNA197269PRJNA186683

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE43549 | GEO | 2013/08/28

SECONDARY ACCESSION(S): PRJNA186683

REPOSITORIES: GEO

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