Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Normal placenta

ABSTRACT: Total RNA was isolated from all the placental tissues using Trizol, and mRNA was isolated using FastTrack Kit. We used a placenta reference as a standard for all these array hybridizations. The placenta reference is a mixture of placenta mRNA with CRG. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

ORGANISM(S): Homo sapiens

PROVIDER: GSE4421 | GEO | 2006/03/09

SECONDARY ACCESSION(S): PRJNA94615

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Normal placenta

Project description:Total RNA was isolated from all the placental tissues using Trizol, and mRNA was isolated using FastTrack Kit. We used a placenta reference as a standard for all these array hybridizations. The placenta reference is a mixture of placenta mRNA with CRG. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Computed

2010-07-01 | E-GEOD-4421 | biostudies-arrayexpress

Transcription profiling of human placenta vs a placenta reference

2006-09-15 | E-SMDB-985 | biostudies-arrayexpress

Basal Cell Carcinoma Tumor-derived Fibroblasts

Project description:Dataset contains samples that are all fibroblast cells that have been cultured from fresh human tissue. Fibroblasts were derived either from tumor (basal cell carcinoma) or non-tumor (control) tissue. Samples were maintained in DMEM, 10% FBS, glutamine, pen/strep and passaged for 4 passages before RNA harvest was performed using Invitrogen FastTrack. Reference channel RNA is the same for each array in the dataset and is Stratagene Universal Reference RNA. Hybridizations were performed for 14-18 hours at 65 C. Scanning was performed using AxonScanner and data were analyzed using GenePix 3.0. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Disease State: Tumor-derived Keywords: Logical Set

2006-10-05 | GSE5502 | GEO

Normal_human_tissues_genomic_controls

Project description:Array hybridizations profiling diverse human tissues; for each array the human tissue RNA is hybridized against a universal reference RNA. Experiments 1-3 are replicates of universal reference RNA vs. genomic DNA hybridizations, used to estimate transcript abundance. Arrays 4-8 are tissue RNA vs. genomic DNA hybridizations, carried out to evaluate the method for estimating transcript abundance (see manuscript for details). Logical set Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: other

2005-02-21 | GSE2194 | GEO

Normal_human_tissues

Project description:Array hybridizations profiling diverse human tissues; for each array the human tissue RNA is hybridized against a universal reference RNA. Series_type: Logical set Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: other

2005-02-21 | GSE2193 | GEO

Niemann-Pick type C fibroblast analysis

Project description:Primary skin fibroblasts from four Niemann-Pick type C patients homozygous for the I1061T mutation and four control individuals were cultured under identical conditions in DMEM containing 10% fetal bovine serum. Cells were harvested at 50-70% confluency. mRNA was isolated with the FastTrack 2.0 mRNA isolation kit according to the manufacturer's instructions (Invitrogen, Carlsbad, CA). A reference RNA comprised of 10 cell lines was used as the control for each hybridized sample (Stratagene, La Jolla, CA). Both sample and reference RNAs were amplified using the MessageAmp II aRNA amplification kit (Ambion, Austin, TX). Set of arrays that are part of repeated experiments Keywords: Biological Replicate

2007-08-15 | GSE8770 | GEO

Normal_human_tissues_genomic_controls

2010-06-10 | E-GEOD-2194 | biostudies-arrayexpress

Arthritis and Rheumatism

Project description:Synovial tissue mRNA from 21 RA patients and 9 OA patients is hybridized against a common reference sample on lymphochips. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

2005-12-17 | GSE3848 | GEO

A DNA microarray survey of gene expression in normal human tissues

2005-02-21 | E-GEOD-2193 | biostudies-arrayexpress