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Isolation of human skeletal muscle precursor cells by fluorescence-activated cell sorting.

ABSTRACT: Novel fluorescence-activated cell sorting (FACS) strategies to prospectively purify functionally distinct cell populations from the human myofiber-associated (hMFA) cell compartment, including human Skeletal Muscle Precursor cells (hSMPs): HSMPs, identified as CD45-Mac1-GlyA-CD31-CD34-CD56intITGA7hi hMFA cells, are highly enriched for cells expressing the satellite cell marker PAX7 and show efficient myogenic and lack adipogenic capacity. CD45-CD11b-GlyA-CD31-CD34+ hMFA cells (CD34+ cells) do not express PAX7, lack myogenic and exhibit adipogenic activity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE44227 | GEO | 2014/01/08

SECONDARY ACCESSION(S): PRJNA189207

REPOSITORIES: GEO

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Isolation of human skeletal muscle precursor cells by fluorescence-activated cell sorting.

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