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Gene expression analysis in Drosophila introgression hybrids

ABSTRACT: RNA was extracted from adult male and adult female Drosophila simulans carrying small genomic segments introgressed from Drosophila mauritiana

ORGANISM(S): Drosophila mauritiana Drosophila simulans Drosophila melanogaster

PROVIDER: GSE44329 | GEO | 2013/02/15

SECONDARY ACCESSION(S): PRJNA189475

REPOSITORIES: GEO

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Gene expression analysis in Drosophila introgression hybrids

Project description:RNA was extracted from adult male and adult female Drosophila simulans carrying small genomic segments introgressed from Drosophila mauritiana Seven introgression genotypes were profiled, as well as the parental D. simulans and D. mauritiana strains. Each sex-by-genotype was assayed on four replicate arrays incorporating a dye swap

2013-02-15 | E-GEOD-44329 | biostudies-arrayexpress

Fine scale genetic mapping of a Hybrid Male Sterility factor between Drosophila simulans and D. mauritiana

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Fine scale genetic mapping of a Hybrid Male Sterility factor between Drosophila simulans and D. mauritiana

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Proteome Plasticity in Drosophila Hybrids

Project description:This submission contains 3 different datasets: - series A: D. melanogaster female ? D. simulans male embryos vs internal standard of pooled D. simulans and D. melanogaster (1:1) embryos - series B: D. simulans female ? D. melanogaster male embryos vs internal standard of pooled D. simulans and D. melanogaster (1:1) embryos - series E: D. melanogaster female ? D. simulans male adult heads vs internal standard of pooled D. simulans and D. melanogaster (1:1) adult heads Each dataset is in triplicates. Quantitation was performed with isobaric isotopologues labelling.

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Comparison of testes gene expression of D. melanogaster species and their hybrids

2006-06-12 | GSE3673 | GEO

Comparison of testes gene expression of D. melanogaster species and their hybrids

Project description:Males hybrids from the crosses between species of the D. simulans clade are steriles as the females are fertiles. Hybrid male sterility is due to severe defects in spermatogenesis and phenotypic differences are observed between the different hybrids involving D. simulans, D. sechellia and D. mauritiana. In this study we are comparing gene expression in the testes of hybrids involving the female D. simulans and the males D. melanogaster, D. mauritiana, or D. sechellia to the gene expression in species testes. Keywords: Comparative genomic hybridization 4 species (D. melanogaster, D. simulans, D. sechellia, D. mauritiana) and 3 different hybrids were used in this study. RNA extracted from whole D. melanogaster males was used as a reference. Hybridizations were perfomed using RNA extraxted from a pool of 200 testes from a sample with RNA extracted from whole D. melanogaster males. At least three independent replicates per hybridizations were performed

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Drosophila simulans complex

Project description:Species of the Drosophila simulans complex (Drosophila simulans, Drosophila mauritiana, Drosophila sechellia) Raw sequence reads

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Drosophila

Project description:Genome sequences and assemblies of Drosophila simulans, Drosophila sechellia and Drosophila mauritiana.

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Comparison of D. simulans/D. sechellia third instar male larvae hybrid expression values to pure-species larvae parents

Project description:Species often produce sterile hybrids early in their evolutionary divergence, and some evidence suggests that hybrid sterility may be associated with deviations or disruptions in gene expression. In support of this idea, many studies have shown that a high proportion of male-biased genes are underexpressed compared to non-sex-biased genes in sterile F1 male hybrids of Drosophila species. In this study, we examined and compared patterns of misexpression in F1 hybrid male third instar larvae of Drosophila simulans and its sibling species, D. sechellia. We analyzed hybrids using custom cDNA arrays we developed from RT-PCRs of spermatogenesis-related transcripts from these species and another sibling species (D. mauritiana). The results from a commercial genome-wide array and custom chip for adults of this species pair, from the custom chip and the genome-wide chip for adults of the D. simulans-D. mauritiana species pair, and from the larvae of the D. simulans-D. mauritiana species pair, are presented separately. Keywords: Comparison of pure-species Drosophila expression to hybrid expression in larvae

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