Project description:doxorubicin is commonly used for hematological malignancies. Since some of PTCL patients resistance to dox and die due to refractory disease or relapse, enhanced knowledge about drug response mechanisms is required to improve treatment outcome. we found an increased expression of HDAC3 in T lymphoma H9 cells following dox exprosure, we compared the gene expression profiles of H9 cells pre/post treated with doxorubicin to explore the potential mechanism of the high expression of HDAC3
Project description:In order to explore the role of LHX2 in neural fate determination, we performed knockdown and overexpression studies in H9. --doxycycline induced LHX2 overexpression in H9-- GFP as a control 3 days doxycyline treatment was performed samples was collected 1 day (Day4) and 13 days (Day16) post doxycyline treatment --shLHX2 in H9-- shLuc as a control samples were collected 12 days of neural induction all samples were biological duplicate