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Gene expression in hTERT-RPE1 cells with overexpression of MFRP

ABSTRACT: Gene expression was compared between hTERT-RPE1 cells and hTERT-RPE1 cells stably overexpressing mouse MFRP with an N-terminal GFP fusion.

ORGANISM(S): Homo sapiens

PROVIDER: GSE44540 | GEO | 2014/03/01

SECONDARY ACCESSION(S): PRJNA190036

REPOSITORIES: GEO

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Gene expression in hTERT-RPE1 cells with overexpression of MFRP

Project description:Gene expression was compared between hTERT-RPE1 cells and hTERT-RPE1 cells stably overexpressing mouse MFRP with an N-terminal GFP fusion. RNA was prepared from hTERT-RPE1 cells overexpressing GFP-MFRP and control cells. Both conditions were done in triplicate. Affymetrix GeneChip Human Genome U133Plus2.0 arrays were used to interrogate changes in gene expression. Image data were quantified with Affymetrix Expression Console Software and normalized with Robust Multichip Analysis.

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