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Bovine tissue gene expression profiling at peak lactation

ABSTRACT: Tissue from mammary, liver, and three adipose depots (mesenteric, omental, and subcutaneous) was collected at slaughter from a cow at peak lactation to examine gene expression profiles of 7872 sequences using a bovine cDNA microarray. Keywords: comparative tissue gene expression

ORGANISM(S): Bos taurus

PROVIDER: GSE4495 | GEO | 2006/03/21

SECONDARY ACCESSION(S): PRJNA94375

REPOSITORIES: GEO

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Bovine tissue gene expression profiling at peak lactation

Project description:Tissue from mammary, liver, and three adipose depots (mesenteric, omental, and subcutaneous) was collected at slaughter from a cow at peak lactation to examine gene expression profiles of 7872 sequences using a bovine cDNA microarray. RNA (20 ug) from tissues and a reference standard derived from a mixture of tissues were used to make aminoallyl-labeled cDNA followed by incorporation of Cy3-ester and Cy5-ester (Amersham, Piscataway, NJ). Each experimental sample was co-hybridized with the reference standard, which allowed the treatment of fluorescence ratios as measurements of relative expression across all samples and time points. Probes were hybridized to the array for 2 days at 42 C. All samples were hybridized to duplicate slides for a total of 4 spots per cDNA element. The microarray platform has already been described [Everts et al. (2005) Vet. Immunol. Immunopathol. 105:235-245]. Slides were scanned for both dye channels with a Scanarray 4000 (GSI-Lumonics, Billerica, MA) dual-laser confocal scanner and images were processed using GenePix 4.0 (Axon Instruments, Inc., CA).

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Real-time quantitative PCR analysis of goat mammary glands in peak-lactation and late-lactation periods

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Real-time quantitative PCR analysis of goat mammary glands in peak-lactation and late-lactation periods

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Gene expression analysis of omental and mesenteric adipose tissue in Crohn's disease and ulcerative colitis

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The transcriptomic profile of peripheral blood nuclear cells in bovine and cervine in peak lactation and dry period

Project description:The aim of the study was to identify genes which are differentially expressed in the peripheral blood nuclear cells of two breeds of cattle (Holstein-Friesian and Polish Red) and cervine in different points in their physiological states (dry-off period, peak of lactation) RNA from peripheral blood nuclear cells taken from cattle and cervine in peak lactation and dry period were hybridized to Agilent two color microarrays with a common reference. There were four Holstein-Friesian cattle, four Polish Red cattle and four deer investigated. The whole blood was drawn in two time point from each animal – during dry period and peak lactation. This means that there were six research groups (Holstein-Friesian cattle in dry period and Holstein-Friesian cattle in peak lactation; Polish Red cattle in dry period and Polish Red cattle in peak lactation; Deer in dry period and Deer in peak lactation). Using Gene Spring Software (one-way ANOVA and Tukey's HSD Post-hoc test) three lists of differentially expressed transcripts were obtained: a list of 576 transcripts which differ deer in dry period and in peak lactation, a list of 437 transcripts which differ Holstein-Friesian cattle in dry period and in peak lactation and a list of 158 transcripts which differ Polish Red cattle in dry period and in peak lactation.

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The transcriptomic profile of peripheral blood nuclear cells in bovine and cervine in peak lactation and dry period

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Transcriptomics comparisons of Mac-T cells versus mammary tissue during late pregnancy and peak lactation

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