Genomics

Dataset Information

0

Genome-wide maps of chromatin state for H3.3 tagged cells


ABSTRACT: The HIRA chaperone complex, comprised of HIRA, UBN1 and CABIN1, collaborates with histone-binding protein ASF1a to incorporate histone variant H3.3 into chromatin in a DNA replication-independent manner. To better understand its function and mechanism, we integrated HIRA, UBN1, ASF1a and histone H3.3 ChIP-seq and gene expression analyses. Most HIRA-binding sites co-localize with UBN1, ASF1a and H3.3 at active promoters and active and weak/poised enhancers. At promoters, binding of HIRA/UBN1/ASF1a correlates with the level of gene expression. HIRA is required for deposition of histone H3.3 at its binding sites. There are marked differences in nucleosome and co-regulator composition at different classes of HIRA-bound regulatory site. Underscoring this, we report novel physical interactions between the HIRA complex and transcription factors, a chromatin insulator and an ATP-dependent chromatin-remodelling complex. Our results map the distribution of the HIRA chaperone across the chromatin landscape and point to different interacting partners at functionally distinct regulatory sites.

ORGANISM(S): Homo sapiens

PROVIDER: GSE45023 | GEO | 2013/04/18

SECONDARY ACCESSION(S): PRJNA192851

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-04-18 | E-GEOD-45024 | biostudies-arrayexpress
2013-04-18 | E-GEOD-45023 | biostudies-arrayexpress
2013-04-18 | E-GEOD-45022 | biostudies-arrayexpress
2013-04-18 | GSE45024 | GEO
2013-04-18 | GSE45022 | GEO
2013-03-20 | E-GEOD-45295 | biostudies-arrayexpress
2013-03-20 | GSE45295 | GEO
2021-07-14 | PXD025466 | Pride
2018-08-23 | GSE117034 | GEO
2018-08-23 | GSE117032 | GEO