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NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata

ABSTRACT: Identification of significantly altered transcripts in Nicotiana attenuata plants with reduced MYC2 expression after induction with oral secretions of Manduca sexta larvae.

ORGANISM(S): Nicotiana attenuata

PROVIDER: GSE45608 | GEO | 2013/05/17

SECONDARY ACCESSION(S): PRJNA195395

REPOSITORIES: GEO

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NaMYC2 transcription factor regulates a subset of plant defense responses in Nicotiana attenuata

Project description:Identification of significantly altered transcripts in Nicotiana attenuata plants with reduced MYC2 expression after induction with oral secretions of Manduca sexta larvae. Gene expression in leaves of Nicotiana attenuata EV and MYC2_VIGS plants was measured at 1 hour after induction with oral secretions of Manduca sexta larvae. Three independent experiments were performed with EV plants and three independent experiments were performed with MYC2_VIGS plants. A total of 47 genes were identified as differentially regulated in MYC2_VIGS plants compared to EV plants.

2013-05-17 | E-GEOD-45608 | biostudies-arrayexpress

NaJAZh regulates a subset of defense responses against herbivores and counteracts developmental necrosis in Nicotiana attenuata plants

Project description:Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of JAZh after elicitation with oral secretions of Manduca sexta larvae. Gene expression in leaves of Nicotiana attenuata wild type and irJAZh plants was measured at 2 hour after elicitation with oral secretions of Manduca sexta larvae. Three independent experiments were performed with wild type plants and three independent experiments were performed with irJAZh plants. A total of 480 genes were identified as more the 2 fold differentially regulated in irJAZh plants compared to wild type plants.

2012-07-11 | E-GEOD-33681 | biostudies-arrayexpress

NaJAZh regulates a subset of defense responses against herbivores and counteracts developmental necrosis in Nicotiana attenuata plants

2012-07-12 | GSE33681 | GEO

Characterization of Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 (ir-lecRK1 plants).

Project description:Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 after elicitation with oral secretions of Manduca sexta larvae. Gene expression in leaves of Nicotiana attenuata wild type and ir-lecRK1 plants was measured at 1 hour after elicitation with oral secretions of Manduca sexta larvae. Three independent experiments were performed with wild type plants and three independent experiments were performed with ir-lecRK1 plants. A total of 148 genes were identified as differentially regulated in ir-lecRK1 plants compared to wild type plants.

2011-11-23 | E-GEOD-29905 | biostudies-arrayexpress

Characterization of Nicotiana attenuata plants with reduced expression of lectin receptor kinase 1 (ir-lecRK1 plants).

2011-11-23 | GSE29905 | GEO

A jasmonate ZIM-domain protein NaJAZd regulates floral jasmonic acid levels and counteracts flower abscission in Nicotiana attenuata plants

Project description:Identification of transcripts that change expression in Nicotiana attenuata plants with reduced expression of JAZd after elicitation with oral secretions of Manduca sexta larvae.

2014-12-31 | GSE43395 | GEO

Dicer-like proteins and their role in plant-herbivore interactions in Nicotiana attenuata

Project description:DICER-like proteins produce small RNAs that silence genes involved in development and defenses against viruses and pathogens. Which DCLs participate in plant-herbivore interactions remains unstudied. We identified four distinct DCL genes and stably silenced their expression by RNAi in Nicotiana attenuata, a model system for the study of plant-herbivore interactions. Silencing DCL1 expression was lethal to the plants. Manduca sexta larvae performed significantly better on ir-dcl3and ir-dcl4 plants, but not on ir-dcl2 plants compared to wild type plants. Phytohormones, defense metabolites and microarray analyses revealed that when DCL3 and DCL4 were silenced separately, herbivore resistance traits were regulated in distinctly different ways. Crossing of the lines revealed complex interactions in the patterns of regulation. Single ir-dcl4 and double ir-dcl2/ ir-dcl3 plants were impaired in JA accumulation, while JA-Ile was increased in ir-dcl3 plants. Ir-dcl3 and ir-dcl4 plants were impaired in nicotine accumulation; silencing DCL2 in combination with either DCL3 or DCL4 restored nicotine levels to those of WT. Trypsin proteinase inhibitor activity and transcripts were only silenced in ir-dcl3 plants. We conclude that DCL2/3/4 interact in a complex manner to regulate anti-herbivore defenses and that these interactions significantly complicate the already challenging task of understanding smRNA function in the regulation of biotic interactions. Gene expression in leaves of Nicotiana attenuata wild type, irDCL3 and irDCL4 plants was measured at 1 hour after elicitation with oral secretions of Manduca sexta larvae. Three independent experiments were performed with wild type plants and three independent experiments were performed with irDCL3 and irDCL4 plants. A total of 811 genes were identified as differentially regulated in irDCL3 and irDCL4 compared to wild type plants.

2013-12-24 | E-GEOD-30124 | biostudies-arrayexpress

Tissue specific diurnal rhythm of transcripts and their regulation during herbivore attack in Nicotiana attenuata

Project description:Idenfication and characterization of oscillating transcripts after elicitation with oral secretions from the sepcialist herbivore, Manduca sexta larvae

2011-12-29 | GSE30287 | GEO

SuperSAGE analysis of the Nicotiana attenuata transcriptome after wounding

Project description:Fatty acid-amino acid conjugates (FACs) in the oral secretion (OS) of the Manduca sexta larvae are necessary and sufficient to elicit the herbivory-specific responses in Nicotiana attenuata, an annual wild tobacco species. We used SuperSAGE combined with 454 sequencing to quantify the early transcriptional changes elicited by the FAC N-linolenoyl-glutamic acid (18:3-Glu) in N. attenuata. The second fully expanded leaf of rosette stage plants were wounded by rolling a fabric pattern wheel three times on each side of the midvein and the wounds were immediately supplied with either 20 uL of 0.02% (v/v) Tween-20/water (solvent control) or 10 uL of synthetic N-linolenoyl-glutamic acid (18:3-Glu; 0.03 nmol/ÂµL in 0.02% (v/v) Tween-20/water). Tissue was collected after 30 min of the treatments and frozen in liquid nitrogen. Total RNA was extracted by the phenol/chloroform-LiCl method and poly(A)+-RNA was purified from total RNA. Subsequent steps for the construction of the SuperSAGE libraries and 454 sequencing were performed as previously described using double NlaIII digestions (Molina et al., 2008, BMC Genomics, 9:553). Two SuperSAGE libraries were generated after wounding and FAC elicitation of Nicotiana attenuata leaves. Leaves were harvested after 30 min of the treatments, total RNA extracted, polyA+ mRNA isolated and used for cDNA synthesis and SuperSAGE analysis as described my Matsumura et al (2003).

2010-10-05 | E-GEOD-18595 | biostudies-arrayexpress

Herbivore-induced vaccination of N. attenuata and S. nigrum

Project description:Nicotiana attenuata and Solanum nigrum were challenged with different insects: Manduca sexta and Tupiocoris notatus in different combinations Goals of the study are first to haracterize the transcriptional response of two native Solanaceous plants (Nicotiana attenuata and Solanum nigrum) to attack from two herbivorous pests common on Solanaceous crops (Manduca sexta and Tupiocoris notatus). And second to identify genes involved in herbivore vaccination phenomenon. After germination, inbred and genetically characterized lines of N. attenuata DI92 and S. nigrum Sn30 originally collected from southwestern Utah in 1988, and Jena Germany in 2000, respectively, were grown in a greenhouse. Eggs of Manduca sexta (abbreviated M. s.) came from the North Carolina State University - Entomology Insectary and were hatched under 37°C. Nymphs and adults of Tupiocoris notatus (abbreviated T. n.) came from laboratory colonies started in 2000 with individuals collected from our Utah field sites. Plant were challenged with these different insect and combinations of them. All plants for a given treatment were enclosed in glass-and-mesh insect cages to avoid cross-infection. After 24 h or 5 days the herbivores and their frass were removed, and the above ground biomass of each plant was flash-frozen in liquid nitrogen and stored at -80 °C until RNA extraction. Three independent replicate cages (biological replicates) were used for each of the 7 treatments resulting in a total of 21 cages per species. RNA extraction was performed according to the protocol using Trizol. The RNA samples represent a pooled sample from 4 plants grown in a cage. Keywords: Direct comparison

2007-09-01 | GSE8143 | GEO

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