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Transcriptome study of human mammary epithelial cells following exposure to polymeric Eudragit RS nanoparticles

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE45869 | GEO | 2013/08/01

SECONDARY ACCESSION(S): PRJNA196516

REPOSITORIES: GEO

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Transcriptome study of human mammary epithelial cells following exposure to polymeric Eudragit RS nanoparticles

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-08-01 | E-GEOD-45869 | biostudies-arrayexpress

Transcriptome study of human mammary epithelial cells following exposure to polymeric Eudragit RS nanoparticles part 2

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Transcriptome study of human mammary epithelial cells following exposure to polymeric Eudragit RS nanoparticles part 1

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Transcriptome study of human mammary epithelial cells following exposure to polymeric Eudragit RS nanoparticles part 1

Project description:To further study the transcriptome of human mammary epithelial cells (HMEC 184) after exposure to polymeric Eudragit RS nanoparticles (ENP), we investigate whole genome microarray expression to identify genes regulated by exposure or not to ENP. Changes in gene expression in HMEC 184 cells (50M-BM- % confluence) incubated with 25M-BM- M-BM-5g/mL ENPs (70M-BM- nm) for 24M-BM- h, and without incubation (control) were measured. Three biological replicates were performed as controls (US_01; US_02; US_04) and ENP exposed ones (US_6; US_07; US_08).

2013-08-01 | E-GEOD-45598 | biostudies-arrayexpress

Transcriptome study of human mammary epithelial cells following exposure to polymeric Eudragit RS nanoparticles part 2

Project description:To further study the transcriptome of human mammary epithelial cells (HMEC 184) after exposure to polymeric Eudragit RS nanoparticles (ENP), we investigate whole genome microarray expression to identify genes regulated by exposure or not to ENP. Changes in gene expression in HMEC 184 cells (90M-BM- % confluence) incubated with 25M-BM- M-BM-5g/mL ENPs (70M-BM- nm) for 24M-BM- h, and without incubation (control) were measured. Three biological replicates were performed as controls (US_09; US_10; US_11) and ENP exposed ones (US_12; US_13; US_14).

2013-08-01 | E-GEOD-45868 | biostudies-arrayexpress

Transcriptome study of human mammary epithelial cells following exposure to polymeric Eudragit RS nanoparticles

Project description:Transcriptome study of human mammary epithelial cells following exposure to polymeric Eudragit RS nanoparticles

| PRJNA196516 | ENA

Transcriptome study of Caco-2 human intestinal cells following exposure to empty or S-nitrosoglutathione-loaded polymeric nanoparticles

Project description:To further study the transcriptome of Caco-2 human colon epithelial-like cells after exposure to S-nitrosoglutathione (GSNO, 1.4 μM), or Eudragit RL PO polymeric nanoparticles (NP-ERL, 50 μg/mL) or GSNO loaded nanoparticles (NP-GSNO, 50 μg/mL corresponding to (1.4 μM GSNO) we investigate whole genome microarray to identify genes regulates by exposure or not to GSNO (1.4 μM) or Eudragit RL PO polymeric nanoparticles (NP-ERL, 50 μg/mL) or GSNO loaded nanoparticles (NP-GSNO, 50 μg/mL corresponding to (1.4 μM GSNO).

2015-12-31 | GSE69551 | GEO

Transcriptome study of Caco-2 human intestinal cells following exposure to empty or S-nitrosoglutathione-loaded polymeric nanoparticles

Project description:To further study the transcriptome of Caco-2 human colon epithelial-like cells after exposure to S-nitrosoglutathione (GSNO, 1.4 Î¼M), or Eudragit RL PO polymeric nanoparticles (NP-ERL, 50 Î¼g/mL) or GSNO loaded nanoparticles (NP-GSNO, 50 Î¼g/mL corresponding to (1.4 Î¼M GSNO) we investigate whole genome microarray to identify genes regulates by exposure or not to GSNO (1.4 Î¼M) or Eudragit RL PO polymeric nanoparticles (NP-ERL, 50 Î¼g/mL) or GSNO loaded nanoparticles (NP-GSNO, 50 Î¼g/mL corresponding to (1.4 Î¼M GSNO). Changes in gene expression in Caco-2 cells incubated without (control) or with GSNO or nanoparticles for 4 h, were measured. Four biological replicates were performed as controls: S46_1_4 ; S46_1_3 ; S35_1_4 ; S35_1_3. Four biological replicates were performed for each conditions : wtih GSNO (1.4 ÂµM) exposed cells (S46_2_2 ; S46_2_1 ; S35_2_2 ; S35_2_1), with NP-ERL (50 Î¼g/mL) exposed cells (S46_1_2 ; S46_1_1 ; S35_1_2 ; S35_1_1) with NP-GSNO (50 Î¼g/mL corresponding to 1.4 ÂµM GSNO) exposed cells (S46_2_4 ; S46_2_3 ; S35_2_4 ; S35_2_3)

2015-12-31 | E-GEOD-69551 | biostudies-arrayexpress

Unique growth pattern of human mammary epithelial cells induced by polymeric nanoparticles.

Project description:Due to their unique properties, engineered nanoparticles (NPs) have found broad use in industry, technology, and medicine, including as a vehicle for drug delivery. However, the understanding of NPs' interaction with different types of mammalian cells lags significantly behind their increasing adoption in drug delivery. In this study, we show unique responses of human epithelial breast cells when exposed to polymeric Eudragit® RS NPs (ENPs) for 1-3 days. Cells displayed dose-dependent increases in metabolic activity and growth, but lower proliferation rates, than control cells, as evidenced in tetrazolium salt (WST-1) and 5-bromo-2'-deoxyuridine (BrdU) assays, respectively. Those effects did not affect cell death or mitochondrial fragmentation. We attribute the increase in metabolic activity and growth of cells culture with ENPs to three factors: (1) high affinity of proteins present in the serum for ENPs, (2) adhesion of ENPs to cells, and (3) activation of proliferation and growth pathways. The proteins and genes responsible for stimulating cell adhesion and growth were identified by mass spectrometry and Microarray analyses. We demonstrate a novel property of ENPs, which act to increase cell metabolic activity and growth and organize epithelial cells in the epithelium as determined by Microarray analysis.

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Transcriptomic study of NR8383 cells following exposure to zinc oxide nanoparticles NM110

Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanoparticle exposure, we performed microarray analyses on NR8383 macrophages exposed for 4 h to 2 µg/mL of NP ZnO (20 % cell viability). This project (SmartNanoTox) has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 686098.

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