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Nasonia Tiling Microarray Experiments for Expression Profiling Sexual Development (Larva)

ABSTRACT: A key feature of Nasonia are their form of sex determination, called haplodiploidy. Females are diploid and develop from fertilized eggs, whereas males are haploid and develop parthenogenetically from unfertilized eggs. This form of sex determination is exploited by this experiment, by knowing the sex of the organisms from the earliest stages of development, so to trace the transcriptomes of sexual development of an insect. We conducted three replicates each using RNA from independent biological extractions of male and female early embryo (0-10 hrs), late embryo (18-30 hrs), 1st instar larvae, and pupae. Additional experiments were performed comparing transcription in adult males, adult females, testis and the female reproductive tract.

ORGANISM(S): Nasonia vitripennis

PROVIDER: GSE46080 | GEO | 2018/04/15

REPOSITORIES: GEO

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