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Phenotypic activation and repression of three ELAV-like proteins in Trypanosoma brucei

ABSTRACT: Three potential ELAV-like proteins of T. brucei, including Tb927.3.2930, Tb927.7.5380, and Tb927.8.6650, were either inhibited by RNAi or phenotypically activated by over-expression, followed by microarray analysis of the transcriptome. The results indicated that these ELAV-like proteins regulate the abundance of a large number of T. brucei transcripts, potentially through regulation of mRNA stability.

ORGANISM(S): Trypanosoma brucei Trypanosoma brucei brucei TREU927

PROVIDER: GSE46161 | GEO | 2013/04/18

SECONDARY ACCESSION(S): PRJNA197394

REPOSITORIES: GEO

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Phenotypic activation and repression of three ELAV-like proteins in Trypanosoma brucei

Project description:Three potential ELAV-like proteins of T. brucei, including Tb927.3.2930, Tb927.7.5380, and Tb927.8.6650, were either inhibited by RNAi or phenotypically activated by over-expression, followed by microarray analysis of the transcriptome. The results indicated that these ELAV-like proteins regulate the abundance of a large number of T. brucei transcripts, potentially through regulation of mRNA stability. Each of the three ELAV-like proteins were either inhibited by RNAi or over-expressed, in stable transgenic procyclic form cell lines. Total RNA was extracted 48h after tetracycline induction of the constructs (except for total RNA from Tb927.8.6650 RNAi which was extracted 24h after tet-induction), and sent to NimbleGen for cDNA synthesis and hybridization. Non-induced cells were analyzed in parallel.

2013-04-18 | E-GEOD-46161 | biostudies-arrayexpress

RIP-Seq analysis of Trypanosoma brucei ELAV-like protein Tb927.8.6650

2013-04-18 | GSE46160 | GEO

RIP-Seq analysis of Trypanosoma brucei ELAV-like protein Tb927.8.6650

Project description:A tagged ectopic version of the ELAV-like protein Tb927.8.6650 of T. brucei was expressed in stable cell lines and pulled down. Co-purifying transcripts were analyzed by sequencing to identify RNAs associated with Tb927.8.6650. Stable procyclic form cell lines expressing tetracycline-inducible TAP-tagged Tb927.8.6650 were created. Cells were harvested 48h after tet-induction, followed by tandem affinity purification of Tb927.8.6650, extraction of co-purified RNA, and sequencing.

2013-04-18 | E-GEOD-46160 | biostudies-arrayexpress