Comparing gene expression in CD5+CD1dhigh and CD5-CD1dhigh B cells
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ABSTRACT: We have previously observed that regulatory B cells expressing the apoptosis-inducing surface molecule Fas ligand were enriched in the B cell fraction denoted by a surface phenotype of CD5+CD1dhigh. To identify potential growth factors, transcriptional regulators, or surface markers that might better characterize regulatory B cells, we compared global gene expression by microarray in two B cells subsets isolated by FACS. Splenocytes from 20 DBA/1 male mice were harvested, pooled, and CD19+ B cells were isolated by positive selection using MACS. B cells were then stained for CD5 and CD1d and sorted by FACS into CD5+CD1dhigh and CD5-CD1dhigh populations.
ORGANISM(S): Mus musculus
PROVIDER: GSE46245 | GEO | 2013/06/30
SECONDARY ACCESSION(S): PRJNA198455
REPOSITORIES: GEO
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