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Identification of miRNAs regulated by TGF-b in human CD8+ T cells (small RNA-seq)

ABSTRACT: The goal of the current study was to identify miRNAs regulated by TGF-b in human CD8+ T cells and analyze the function of these miRNAs in shaping the immunomodulatory effect of TGF-b in these cells. We identified the miR-23a cluster to be upregulated and found that this cluster could target key molecules (IFN-g and LAMP1) involved in immune response by CD8+ T cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE46322 | GEO | 2014/08/01

SECONDARY ACCESSION(S): PRJNA198733

REPOSITORIES: GEO

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Identification of miRNAs regulated by TGF-b in human CD8+ T cells (expression)

2014-08-01 | GSE46324 | GEO

Identification of miRNAs regulated by TGF-b in human CD8+ T cells (expression)

Project description:The goal of the current study was to identify miRNAs regulated by TGF-b in human CD8+ T cells and analyze the function of these miRNAs in shaping the immunomodulatory effect of TGF-b in these cells. We identified the miR-23a cluster to be upregulated and found that this cluster could target key molecules (IFN-g and LAMP1) involved in immune response by CD8+ T cells. CD8+ T cells were isolated and purified from healthy human peripheral blood of 5 donors and were activated using beads coupled to anti-CD2, anti-CD3 and anti-CD24. They were then treated with 5ng/ml TGF-b, with 1M-BM-5M SD-208 or left untreated. RNA from these cells were then isolated and used for generating miRNA microarrays.

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Identification of miRNAs regulated by TGF-b in human CD8+ T cells (small RNA-seq)

Project description:The goal of the current study was to identify miRNAs regulated by TGF-b in human CD8+ T cells and analyze the function of these miRNAs in shaping the immunomodulatory effect of TGF-b in these cells. We identified the miR-23a cluster to be upregulated and found that this cluster could target key molecules (IFN-g and LAMP1) involved in immune response by CD8+ T cells. CD8+ T cells were isolated and purified from healthy human peripheral blood of 5 donors and were activated using beads coupled to anti-CD2, anti-CD3 and anti-CD24. They were then treated with 5ng/ml TGF-b, with 1ÂµM SD-208 or left untreated. RNA from these cells were then isolated and used for deep sequencing.

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Integrating CRISPR knockout, interference, and activation to dissect the functional role and underlying signaling of microRNA-23a-27a-24-2 cluster in hepatocellular carcinoma

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Differentially expressed miRNAs in TGF-β1-knock down human colorectal cancer cell lines vs controls

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Differentially expressed miRNAs in TGF-M-NM-21-knock down rhabdmyosarcoma cell lines vs controls

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Differentially expressed miRNAs in TGF-M-NM-21-knock down human colorectal cancer cell lines vs controls

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