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Expression data from neonatal rat ovaries following LPS exposure

ABSTRACT: Inflammatory processes play an important role in regulation of ovarian development, in particularly early in life. Neonatal immune challenge by administration of lipopolysaccharide (LPS) has been previously demonstrated to result in diminished ovarian reserve and altered reproductive lifespan. In this study we aimed to characterise the cellular mechanisms that may underpin impaired ovulatory capacity and reduced oocyte development, induced by LPS treatment. Rat pups were administered with LPS or saline on postnatal days 3 and 5. Ovaries were obtained on postnatal day 7 to examine the effect of LPS administration on the ovarian transcriptome.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE46436 | GEO | 2013/04/27

SECONDARY ACCESSION(S): PRJNA200339

REPOSITORIES: GEO

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Expression data from neonatal rat ovaries following LPS exposure

Project description:Inflammatory processes play an important role in regulation of ovarian development, in particularly early in life. Neonatal immune challenge by administration of lipopolysaccharide (LPS) has been previously demonstrated to result in diminished ovarian reserve and altered reproductive lifespan. In this study we aimed to characterise the cellular mechanisms that may underpin impaired ovulatory capacity and reduced oocyte development, induced by LPS treatment. Rat pups were administered with LPS or saline on postnatal days 3 and 5. Ovaries were obtained on postnatal day 7 to examine the effect of LPS administration on the ovarian transcriptome. Rat pups were administered intraperitoneally either 0.05mg/kg of LPS (Salmonella Enteritidis) or an equivalent volume of non-pyrogenic saline on postnatal days (PNDs) 3 and 5, and ovaries were obtained on PND 7 for RNA extraction and hybridization on an Agilent-028279 SurePrint G3 Rat GE 8x60K Array platform.

2013-04-27 | E-GEOD-46436 | biostudies-arrayexpress

Transcriptome profiling of ovaries in aged mice administered with Samul-tang

Project description:Samul-tang (SM), a traditional herbal medicine is used to treat menstrual irregularities and infertility. The mechanism underlying the role of SM in ovary function needs elucidation. In this study, the influence of SM administration on the ovarian reserve of aged mice was investigated. Female BALB/c mice (8 and 40 weeks-old) were administered with distilled water (young or old group) or SM for 4 weeks. SM administration prevented age-related ovarian follicle loss in mice. Quality of oocytes and blastocysts were enhanced in SM-administrated mice compared to those of non-treated old mice. Further, SM administration increased the pregnancy rate and number of litters. SM triggered changes in aging-related genes that are linked to the RAS-mediated pathway. Thus, we demonstrate that SM can be used to increase the oocyte yield in aged women, potentially improving age-related cognitive decline in the ovarian reserve.

2024-04-30 | GSE264413 | GEO

PRC1-mediated epigenetic programming is required to generate the ovarian reserve

Project description:The ovarian reserve defines the female reproductive lifespan, which in humans spans decades due to robust maintenance of meiotic arrest in oocytes residing in primordial follicles. Epigenetic reprogramming, including DNA demethylation, accompanies meiotic entry, but the chromatin changes that underpin the generation and preservation of ovarian reserves are poorly defined. We report that the Polycomb Repressive Complex 1 (PRC1) establishes repressive chromatin states in perinatal mouse oocytes that directly suppress the gene expression program of meiotic prophase-I and thereby enable the transition to dictyate arrest. PRC1 dysfuction causes depletion of the ovarian reserve and leads to premature ovarian failure. Our study demonstrates a fundamental role for PRC1-mediated gene silencing in female reproductive lifespan, and reveals a critical window of epigenetic programming required to establish ovarian reserve.

2022-06-26 | GSE184208 | GEO

Whole ovaries RNA sequencing; WT and ghrelin o-acyltransferase (GOAT) knockout mice

Project description:Ghrelin, an orexigenic gut-derived peptide, is gaining increasing attention due to its multifaceted role in a number of physiological functions, including metabolism, cardiovascular health, stress and reproduction. Ghrelin exists in circulation primarily as des-acylated and acylated ghrelin. Des-acyl ghrelin, until recently considered to be an inactive form ghrelin, is now known to have independent physiological functionality. However, the relative contribution of acyl and des-acyl ghrelin to reproductive development and function is currently unknown. Here we used ghrelin-O-acyltransferase (GOAT) knockout (KO) mice that have no measurable levels of endogenous acyl ghrelin and chronically high levels of des-acyl ghrelin, to characterise how the developmental and life-long absence of acyl ghrelin affects ovarian development and reproductive capacity. We have combined ovarian transcriptome analysis using RNA sequencing with measures of ovarian morphometry, as well as with the assessment of markers of reproductive maturity and the capacity to breed. Our data show pronounced specific changes in the ovarian transcriptome in the juvenile GOAT KO ovary, indicative of advanced ovarian development. These changes corresponded with diminished ovarian reserve in the juvenile and adult ovaries of these mice, due to a continuous reduction in the number of small follicle populations. These changes did not affect the timing of puberty onset or reproductive capacity under optimal conditions. These data suggest that an absence of acyl ghrelin does not prevent reproductive success but that appropriate levels of acyl and des-acyl ghrelin may be necessary for optimal ovarian maturation.

2019-02-05 | GSE106339 | GEO

Genome-wide maps of H3K27Ac occupancy on chromatin in mouse ovaries before and after hCG treatment.

Project description:Chromatin immunoprecipitation-based sequencing (ChIP-seq) of H3K27Ac was performed using mouse ovarian granulosa cells before and after ovulatory signal stimulation. We found genome-wide H3K27Ac was erased rapidly at 1h after hCG treatment, and new H3K27Ac was established at 4h after hCG treatment.

2021-01-30 | GSE165809 | GEO

High-fat diet exposure, regardless of the induction of obesity, is associated with altered expression of genes critical for normal ovarian function

Project description:High-fat diet (HFD) induces diminished ovarian reserve and subfertility in female mice, irrespective of obesity. To further assess the impact of HFD on ovarian function, female mice were fed a 60% HFD or standard chow for 10 weeks and then HFD fed mice were separated into obese (HFOB) and lean (HFLN) groups. RNA-sequencing of whole ovaries identified multiple differentially expressed genes involved in normal ovarian function (ovulation, luteinization, luteolysis) in both the HFLN and HFOB mice compared to the chow fed mice (q<0.05). Taken together, elevated dietary fat intake, regardless of obesity, is associated with impaired estrous cycles and altered expression of genes critical to normal ovulatory function.

2019-02-12 | GSE101906 | GEO

Global changes in granulosa cell transcription in response to the LH surge

Project description:During ovulation, the LH surge leads to the activation of various signalling cascades in granulosa cells, resulting in a critical shift in chromatin landscape and correspondingly the ovarian gene expression profile. Such large-scale genomic reprogramming involves a specific suite of ovulatory transcription factors. This study aims to characterise global changes in the chromatin landscape that are induced by the LH surge in granulosa cells during ovulation.

2023-06-22 | GSE178314 | GEO

Transcriptomic analysis of 13.5dpc embryonic ovaries exposed to the acetaminophen (APAP) and ibuprofen (IBU) combination in mice

Project description:Nonsteroidal anti-inflammatory drugs such as ibuprofen (IBU) and analgesic drugs, such as acetaminophen (APAP), are the most frequently medications taken by pregnant women, even in combination. They were shown to target fetal gonadal development acting as endocrine disruptors, that might favour genital malformations in new-born boys and reproductive disorders in adults, in an intergenerational manner. However, the consequences on postnatal ovarian development and female reproductive health after in utero exposure are still unknown. Here, we show that in utero exposure to therapeutic doses of the widely used APAP-IBU combination during the sensitive window of sex determination leads to an increased proliferation of female embryonic germ cells and a delayed entry into meiosis in 13.5dpc exposed ovaries. Consequently, the primordial follicle formation is enhanced in postnatal ovaries and is followed by a reduced follicular activation through the Akt/FoxO3 pathway and an increased follicular apoptosis. Subsequently, a reduced ovarian reserve in adult ovaries of exposed animals and in their offspring (F1) is observed. This leads to the subfertility of 6-month-old F1 animals that show an accelerated ovarian aging, a Premature Ovarian Insufficiency-like phenotype, with an abnormal persistence of corpora lutea due to the decreased apoptosis and an increased luteal Akt-mediated cell survival. Our data suggest that the use of APAP+IBU during this critical period of development that occurs during the first trimester of gestation (6 to 10 weeks), could lead in humans to adverse effects that could be passed to the offspring.

2019-08-22 | GSE122547 | GEO

Gene expression changes in ovaries of oocyte-specific conditional knockout of Ubc9 in juvenile mice

Project description:The reproductive lifespan in mammals is largely determined by the abundance and quality of oocytes residing within the primoridial ovarian follicular pool. Critical oocyte-expressed genes driving ovarian function have been identified, buta role for post-translational modifications, such as SUMOylation of these key factors is not well understood. Oocyte-specific genetic ablation of the sole E2 SUMOylation enzyme, UBC9, cause female sterility. Preantral and antral stage ovarian follicles are depleted by sexual maturity, and loss of the ovarian reserve in early adulthood causes premature ovarian failure, a condition associated with infertility in women. Gene expression changes indicate atered function of multiple POI candidate genes, including the transcription factors, NOBOX and SOHLH1. Together, these studies show that SUMOylation is required in the mammalian oocyte for regulating key genetic determinants of oocyte growth and differentiation.

2019-11-15 | GSE133179 | GEO

Transcriptomic Profiling of human granulosa cells between women with advanced maternal age with different ovarian reserves

Project description:Age-related diminished ovarian reserve (DOR) is not absolute. Some advanced maternal age (AMA) still have normal ovarian reserve (NOR) and often show better pregnancy outcomes. Exploring the transcriptomic profile of AMA could lead to new ideas for mitigating age-related diminished ovarian reserve.

2023-05-16 | GSE232306 | GEO

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