Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional profile of Escherichia coli K12 strain JM109 harboring pUCA1501

ABSTRACT: A1501 aroA is a gene derived from Pseudomonas stutzeri A1501, encoding a class II glyphosate-tolerant EPSP synthase. To understand the effect of class II EPSP synthase to E. coli under glyphosate shock, we constructed the class II EPSP synthase-expressing plasmid pUC-A1501. And pUC18 is the empty vector used as a control.

ORGANISM(S): Escherichia coli

PROVIDER: GSE46625 | GEO | 2013/10/15

SECONDARY ACCESSION(S): PRJNA201432

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptional profile of Escherichia coli K12 strain JM109 harboring pUCA1501

Project description:A1501 aroA is a gene derived from Pseudomonas stutzeri A1501, encoding a class II glyphosate-tolerant EPSP synthase. To understand the effect of class II EPSP synthase to E. coli under glyphosate shock, we constructed the class II EPSP synthase-expressing plasmid pUC-A1501. And pUC18 is the empty vector used as a control. RNA extracted from cells of E. coli K-12 JM109 cells with pUCA1501 till OD600 to achieve 0.4, or cells with pUC18 when their OD600 achieved 0.4.

2013-10-15 | E-GEOD-46625 | biostudies-arrayexpress

Transcriptional profile of Escherichia coli K12 strain DH10B harboring A1501 nitrogen fixation island (NFI) under nitrogen fixation conditions

Project description:A1501 NFI is a genomic island derived from Pseudomonas stutzeri A1501. To study the molecular interactions of the P. stutzeri nif genes with the E. coli genome during nitrogen fixation, the NIF of A1501 was transferred into E. coli and comparative transcriptomics analyses were performed between nitrogen fixation conditions and nitrogen excess conditions.

2015-08-05 | GSE37780 | GEO

Transcriptional profile of Escherichia coli K12 strain JM109 under 200 mM glyphosate shock

Project description:Glyphosate (GLY) is an effective antimetabolite that acts against the shikimate pathway 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, However, little is known about the genome-scale transcriptional responses of bacteria after glyphosate shock. To investigate further the mechanisms by which E. coli response to a glyphosate shock, a DNA-based microarray was used for transcriptional analysis of E. coli exposed to 200 mM glyphosate.

2011-07-22 | GSE30838 | GEO

Transcriptional profile of Escherichia coli K12 strain JM109 under 200 mM glyphosate shock

2011-07-21 | E-GEOD-30838 | biostudies-arrayexpress

Expression profiling of P. stutzeri A1501 treated with 20mM ammonia shock for 10 min

Project description:A whole genome DNA microarray was used to undertake a global transcriptional analysis of nitrogen fixation and ammonium repression in Pseudomonas stutzeri A1501. The aim of this study was to identify the genes that are up-regulated under nitrogen fixation conditions and rapidly down-regulated as soon as 10 min after ammonia shock. The expression changed genes may be the candidate genes for the ammonia signal transmission or be involved in the nitrogen regulatory mechanism. First, P. stutzeri A1501 was treated with 0.1 mM ammonia and 0.5% Oxygen tension until the nitrogenase activity was detectable. Then the cells were sudden shifted from the nitrogen fixation conditions to the ammonia repression conditions by addition of 20 mM ammonia for 10min. Subsequently, the bacterium was collected and began the RNA extraction process. Thus, we compare the expression profilings in these two conditions in order to identify the candidate genes.

2010-05-14 | E-GEOD-14775 | biostudies-arrayexpress

Microarray analysis of the wildtype and recombinant E. coli strain containing an ncRNA nfiS to H2O2 shock

Project description:The aim of this study was to investigate the expressional changes profiles of the wildtype and recombinant Escherichia coli strain which contains an non-coding RNA nfiS from the nitrogen-fixing Pseudomonas stutzeri A1501 after hydrogen peroxide shock, so as to lay a theoretical foundation for further clarifying the mechanism of this ncRNA to enhance the resistance ability of E.coli to oxidative stress. In our early research, an ncRNA named NfiS from the nitrogen fixing bacteria Pseudomonas stutzeri A1501 was identified to play an important role in the response to oxidative as well as osmotic stress. In this work, the nifS gene was transferred to E. coli Trans10. Heterologous expression of the nfiS in Trans10 enhanced its tolerance to salt stress and oxidative stress. To further study the effect of nfiS on gene expression in E. coli, microarray assay was performed to delineate the transcriptome difference between nfiS-expressing strain and wild type under H2O2 shock treatment.

2019-10-15 | GSE124807 | GEO

Expression profiling of P. stutzeri A1501 treated with 20mM ammonia shock for 10 min

2010-02-10 | GSE14775 | GEO