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Three-stage continuous culture system with a self-generated anaerobia to study the regionalized metabolism of the human gut microbiota

ABSTRACT: A three-stage continuous fermentative system was developed to simulate and control physicochemical factors of the gut biology. Inoculation was of each reactor was performed from a human fecal sample which was initially amplified with a batch procedure. Samples from the initial feces, the batch and from the bioreactors media were collected to extract bacterial DNA. 16S PCR amplification was performed to assess the microbial diversity at the family level using the HuGChip. Amplified DNA was purified and labelled with either Cy3 or Cy5 dye and hybridized on the microarray.

ORGANISM(S): uncultured bacterium human gut metagenome

PROVIDER: GSE46677 | GEO | 2013/05/07

SECONDARY ACCESSION(S): PRJNA201498

REPOSITORIES: GEO

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