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Identification of the phosphotransacetylase (pta) and acetate kinase (ackA) regulons in Streptococcus mutans UA159

ABSTRACT: Transcriptional comparisons between wild-type Streptococcus mutans UA159 and two mutant strains (∆ackA and ∆ackA pta).

ORGANISM(S): Streptococcus mutans UA159 Streptococcus mutans

PROVIDER: GSE46835 | GEO | 2013/05/11

SECONDARY ACCESSION(S): PRJNA202444

REPOSITORIES: GEO

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Identification of the phosphotransacetylase (pta) and acetate kinase (ackA) regulons in Streptococcus mutans UA159

Project description:Transcriptional comparisons between wild-type Streptococcus mutans UA159 and two mutant strains (M-bM-^HM-^FackA and M-bM-^HM-^FackA pta). On growth condition in defined FMC medium, WT vs. either M-bM-^HM-^FackA or M-bM-^HM-^FackA pta mutants. Biological replicates: 10 control, 5 each mutant, independently grown and harvested. One replicate per array.

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Project description:Identification of the phosphotransacetylase (pta) and acetate kinase (ackA) regulons in Streptococcus mutans UA159

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Project description:Transcriptional analysis of the regulator FabT in S. mutans RNA was extracted from 4 replicate samples of S. mutans UA159 and MU1591 (M-bM-^HM-^FfabT) grown in continuous culture to steady-state pH values of 7 and 5. RNA was labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from S. mutans UA159 cultures grown to mid-log.

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