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PTHR1 knock-down in osteosarcoma cells

ABSTRACT: Expression analysis of OS tumors with shRNA knockdown of PTHR1 mouse OS 80 (a Cre:lox OS cell line from Boston) was tagged with firefly luciferase expression construct. They were then infected with either control (renilla luciferase shRNA) or an shRNA that effective knocks-down PTHR1 (PTHR1.358; para-thyroid hormone receptor). The cells were injected with matrigel onto the back flank of Balb/c nu/nu mice and left to grow for 1 month with weekly monitoring of tumour size by in vivo luciferase assay. At 4 weeks the tumours were removed and trizol stored. Whole tumour was ground up and set form micro-array.

ORGANISM(S): Mus musculus

PROVIDER: GSE46861 | GEO | 2015/07/01

SECONDARY ACCESSION(S): PRJNA202867

REPOSITORIES: GEO

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PTHR1 knock-down in osteosarcoma cells

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