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Expression profile and RNA Polymerase II occupancy of transposable elements among knock down of the piRNA pathway components in OSCs

ABSTRACT: The Piwi–piRNA complex (Piwi–piRISC) in Drosophila ovarian somatic cells represses transposons transcriptionally to maintain genome integrity; however, the underlying mechanisms remain obscure. We performed mRNA-seq analysis from OSCs transfected with siRNAs against CG3893, the known piRNA pathway genes, Piwi, Maelstrom, HP1a and Armitage, and the control (EGFP), and PolII ChIP-seqanalysis from OSCs transfected with siRNAs against CG3893, Piwi, Mael and the control (EGFP). This result indicates that CG3893 is a novel factor for primary piRNA pathway in OSCs.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE47006 | GEO | 2013/08/01

SECONDARY ACCESSION(S): PRJNA203266

REPOSITORIES: GEO

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Expression profile and RNA Polymerase II occupancy of transposable elements among knock down of the piRNA pathway components in OSCs

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