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Nucleosome loss leads to global transcriptional upregulation and genomic instability during yeast replicative aging

ABSTRACT: All eukaryotic cells divide a finite number of times, termed replicative aging, but the reason for this is not clear. Consistent with the decreased total histone protein levels in aged Saccharomyces cerevisiae, which is a cause of aging (1), we find that nucleosome occupancy decreases 50% across the whole genome during replicative aging by spike-in controlled MNase sequencing. Nucleosomes become fuzzier or move to sequences predicted to better accommodate histone octamers. All yeast genes are induced during aging. Genes that are repressed in young cells are most induced, accompanied by nucleosome loss from their promoters that have unique chromatin organization. Contrary to the loss of mitochondrial function during aging, mitochondrial DNA content increases and unprecedented levels of large-scale chromosomal alterations and increased retrotransposition are observed.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE47023 | GEO | 2014/02/26

SECONDARY ACCESSION(S): PRJNA203300

REPOSITORIES: GEO

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Nucleosome loss leads to global transcriptional upregulation and genomic instability during yeast replicative aging

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