Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Evidence for the independent evolution of "functional progesterone withdrawal" in primates and guinea pigs: a comparative transcriptomic study

ABSTRACT: Cervix remodeling (CRM) is a critical process in preparation for parturition. Early cervix shortening is a powerful clinical predictor of preterm birth, and thus understanding how CRM is regulated is important for the prevention of prematurity. Humans and other primates differ from most other mammals by the maintenance of high levels of systemic progesterone concentrations and thus differ dramatically from most model species. Humans have been hypothesized to perform functional progesterone withdrawal (FPW), but the mechanisms of FPW are not known. Guinea pigs are similar to humans as they also maintain high progesterone concentrations through parturition. The aim of this study is to document gene expression during pregnancy in the guinea pig uterine cervix in order to assess whether guinea pig can be used as a model for human FPW

ORGANISM(S): Cavia porcellus

PROVIDER: GSE47986 | GEO | 2013/12/31

SECONDARY ACCESSION(S): PRJNA208534

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Evidence for the independent evolution of "functional progesterone withdrawal" in primates and guinea pigs: a comparative transcriptomic study

Project description:Cervix remodeling (CRM) is a critical process in preparation for parturition. Early cervix shortening is a powerful clinical predictor of preterm birth, and thus understanding how CRM is regulated is important for the prevention of prematurity. Humans and other primates differ from most other mammals by the maintenance of high levels of systemic progesterone concentrations and thus differ dramatically from most model species. Humans have been hypothesized to perform functional progesterone withdrawal (FPW), but the mechanisms of FPW are not known. Guinea pigs are similar to humans as they also maintain high progesterone concentrations through parturition. The aim of this study is to document gene expression during pregnancy in the guinea pig uterine cervix in order to assess whether guinea pig can be used as a model for human FPW Total RNA obtained from isolated cervical tissues from non-pregnant and two genstation stages (mid-term, and late-term but not in labor).

2013-12-31 | E-GEOD-47986 | biostudies-arrayexpress

Evolution of Gene Expression in the Uterine Cervix related to Steroid Signaling: Conserved features in the regulation of cervical remodeling.

Project description:The uterine cervix is the boundary structure between the uterus and the vagina and is a key component of the female reproductive tract for the maintenance of pregnancy and timing of parturition. Here we report on a comparative transcriptomic study of the cervix of four placental mammal species, mouse, guinea pig, rabbit and the basal eutherian mammal, armadillo, Dasypus novemcinctus, and one marsupial species, Monodelphis domestica. Our aim is to investigate the evolution of cervical gene expression as related to putative mechanisms for functional progesterone withdrawal. Our main findings are: 1) The patterns of gene expression found in eutherian species is consistent with the notion that an increase in the ratio of E/P4 signaling strength is critical for cervical remodeling. How the increased E/P4 ratio is achieved, however, is variable between eutherian species. 2) None of the genes related to steroid signaling modulated in eutherian species change expression during opossum gestation, suggesting that the role of P4 signaling in cervical remodeling evolved after the common ancestor of marsupials and placental mammals. 3) A tendency for decreased expression of progesterone receptor co-activators (NCOA1, -2 and -3, and CREBP) towards term is a shared derived feature of eutherians. This finding suggests that parturition is associated with broad scale histone de-acetylation. We tested this hypothesis by performing Western-blotting on mouse cervix and found evidence for a striking degree of histone de-acetylation in labor. This finding may have important implications for the control of premature cervical remodeling and preterm birth in humans.

2017-07-31 | GSE85815 | GEO

Transcriptomic profiling of guinea pigs with NASH and advanced fibrosis

Project description:Non-alcoholic steatohepatitis (NASH) is a serious health challenge affecting millions worldwide, and research advances are restricted by the limited availability of preclinical models recapitulating the complex disease etiology and hepatic histopathology. Uniquely, the diet induced guinea pig model develops NASH with fibrosis resembling human histopathology however, no data is available depicting the guinea pig NASH transcriptome. We provide the first high throughput sequencing results on guinea pig NASH with advanced fibrosis. Transcriptomic profiles in guinea pig NASH clearly separated from controls, and pathways involved in fibrosis, inflammation and lipid metabolism were all highly regulated.

2021-10-06 | GSE158168 | GEO

Repeat tick exposure elicits distinct immune responses in guinea pigs and mice [guinea pig]

Project description:RNA-seq gene expression analysis of guinea pig and mouse skin after tick bites

2023-04-20 | GSE148967 | GEO

Development of oligonucleotide microarray to study global gene expression profile in guinea pigs in response to tuberculosis infection

Project description:In this study we developed a guinea pig oligonucleotide microarray (GPOM) comprising of a total number of 45,220 features including 43,803 valid features from different mammalian species. These features are inclusive of 2971 newly annotated probes corresponding to 344 unique genes of guinea pig. As a case study, we utilized this array to examine the gene expression profile in guinea pig lungs in response to infection with Mycobacterium tuberculosis. Studying the global gene expression profile in guinea pigs allowed identification of the disease signature of pulmonary TB infection represented by several unique genes that are differentially regulated in this model. While, 1344 unique genes exhibited marked up regulation, 1856 genes were significantly down regulated. The newly developed tool not only finds its utility in studying the global gene expression profile associated with vaccination and/or M. tuberculosis infection in this highly useful animal model but would also be immensely useful in identification of new drug targets, testing of therapies, molecular genetic analysis for diseases other than tuberculosis as well. Genotypic Technology designed Custom Cavia porcellus 4x44k Gene Expression Array (Agilent-AMADID-019424) * In order to validate the GPOM developed in this study, we compared the gene expression profile of guinea pig lungs at 10 weeks post- M. tuberculosis infection with respect to that obtained from normal uninfected animals. To address this, guinea pigs were aerosol infected with M. tuberculosis, lungs were harvested at 10 weeks post-infection and RNA obtained from infected lungs was employed for cDNA synthesis and microarray hybridization. The gene expression was compared with the RNA sample obtained from the lungs of normal uninfected guinea pigs.

2012-10-01 | E-GEOD-32447 | biostudies-arrayexpress

Repeat tick exposure elicits distinct immune responses in guinea pigs and mice [mouse]

Project description:RNA-seq gene expression analysis of guinea pig and mouse skin after tick bites

2023-04-20 | GSE148968 | GEO

RNA-seq of ten guinea pig tissues from breeders and non-breeders

Project description:Comparison of gene expression level by Illumina sequencing of ten different guinea pig tissue from breeders and non-breeders.

2018-06-14 | GSE98653 | GEO

Transcriptomics analysis of guinea pig heart, fetus to adult progression

Project description:We report the outcomes of next-generation sequencing (RNA-Seq) of guinea pig (Cavia porcellus) heart tissue and compare relative transcript abundances between fetus and adult.

2018-05-01 | GSE108420 | GEO

Conserved DNA Methylation Signatures in the Prefrontal Cortex of Newborn and Juvenile Guinea Pigs Following Antenatal Betamethasone Exposure

Project description:We investigated the effect of antenatal corticosteroid exposure (ACS) on the DNA methylation patterns in the prefrontal cortex (PFC) of female guinea pig offspring. Guinea pig dams were treated with saline or betamethasone on gestational days 50/51. PFC was collected from newborn (post-natal day 1) and juvenile (PND 14) offspring. Methylation assessment using genomic DNA was performed with reduced representation bisulfite sequencing techniques.

2024-11-06 | GSE280726 | GEO

Identification of a DNA methylation signature in whole blood of newborn guinea pigs and human neonates following antenatal betamethasone exposure

Project description:We investigated the effect of antenatal corticosteroid exposure (ACS) on the DNA methylation patterns in the whole blood of female guinea pig offspring. Guinea pig dams were treated with saline or betamethasone on gestational days 50/51. Whole blood was collected from newborn offspring (post-natal day 1) and dried on blood spot cards. Methylation assessment using genomic DNA was performed with reduced representation bisulfite sequencing techniques.

2024-08-22 | GSE275411 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data