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Exploring the function of the grape regulators VvMYB5a and MYB5b by heterologous expression in anthocyanin petunia mutants

ABSTRACT: To obtain insights about the roles of VvMYB5a and VvMYB5b, here we perform complementation analyses using petunia regulatory mutants impaired in pigment accumulation in flower epidermis, proven to be a valid tool for gene functional studies.

ORGANISM(S): Petunia axillaris Petunia x hybrida

PROVIDER: GSE48058 | GEO | 2013/12/19

SECONDARY ACCESSION(S): PRJNA208825

REPOSITORIES: GEO

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Exploring the function of the grape regulators VvMYB5a and MYB5b by heterologous expression in anthocyanin petunia mutants

Project description:To obtain insights about the roles of VvMYB5a and VvMYB5b, here we perform complementation analyses using petunia regulatory mutants impaired in pigment accumulation in flower epidermis, proven to be a valid tool for gene functional studies. We created three transgenic petunia lines overexpressing VvMYB5a, VvMYB5b and VvMYBA1 and we compared petal transcriptomes of each overexpressors with the untransformed one.

2013-12-19 | E-GEOD-48058 | biostudies-arrayexpress

Diurnal RNA-Seq for Petunia hybrida

Project description:To better understand how diurnal transcriptional regulation contributes to day/night cycles of volatile emission from the petunia flower, RNA extracted from corolla in the morning (7AM) and evening (7PM) was sequenced to determine genes differentially expressed at the two time points.

2023-10-24 | GSE230802 | GEO

Diurnal ChIP-Seq for Petunia hybrida

Project description:To better understand how diurnal transcriptional regulation contributes to day/night cycles of volatile emission from the petunia flower, cross-linked chromatin from corolla in the morning (7AM) and evening (7PM) was immunoprecipitated with antibodies against 4 histone marks associated with trancriptional activity to determine islands enriched for the marks in morning and evening.

2023-11-08 | GSE230857 | GEO

High-throughput sequencing of small RNAs associated with Chalcone Synthase A (CHS-A) sense cosuppression in petunia

Project description:We report the application of sequencing-by-synthesis technology for high-throughput profiling of small RNAs involved in Chalcone synthase A (CHS-A) sense cosuppression in petunia. Sense cosuppression is a classical form of eukaryotic post-transcriptional gene silencing. It was first reported in transgenic petunia, where a transgene overexpressing the host Chalcone Synthase-A (CHS-A) gene caused the degradation of the homologous transcripts and the loss of flower pigmentation. Though sense cosuppression is recognized as an RNA silencing mechanism, little evidence has been yet provided demonstrating its association with the generation of individual small interfering RNAs (siRNAs) that are the assumed determinants. In this work, the deep sequencing of small RNAs in cosuppressed transgenic petunia and WT petunia respectively allowed for the identication of siRNAs that vastly predominate in the silenced flower and guide prominent cleavage events in CHS-A mRNA.

2009-09-28 | GSE13764 | GEO

High-throughput sequencing of small RNAs associated with Chalcone Synthase A (CHS-A) sense cosuppression in petunia

2009-09-27 | E-GEOD-13764 | biostudies-arrayexpress

Phosphoproteome analysis of petunia corolla treated with ethylene and air

Project description:Senescence represents the last stage of flower development. Phosphorylation is one of the key post-translational modifications that regulate protein functions in diverse biological pathways and contexts. Generally, kinases may be more required than phosphatases during plant growth and development. However, little is known about global phosphorylation change during flower senescence. In this work, we quantitatively investigated the petunia phosphoproteome following ethylene or air treatment. In total, 2170 phosphosites in 1184 protein groups were identified, among which 2059 sites in 1124 proteins were quantified. Treatment with ethylene resulted in 711 down-regulated and only 117 up-regulated phosphosites using a 1.5-fold threshold (P<0.05), showing that ethylene negatively regulates global phosphorylation levels and that phosphorylation of lots of proteins was not necessary during flower senescence. Our results show that protein dephosphorylation may play an important role of in ethylene-induced corolla senescence in petunia and that phosphatases may be more required than kinases during flower senescence. In addition, our results show that ethylene regulates ethylene and ABA signaling transduction pathways via phosphorylation level, and plant mRNA splicing machinery was a major target of ethylene-induced dephosphorylation. Moreover, ethylene treatment increases the number of alternative splicing of precursor RNAs in petunia corollas.

2022-02-15 | PXD005599 | Pride

Global proteome of mid- and late-development Petunia inflata flower petal protoplasts

Project description:Establishing the global proteomes of Petunia inflata flower protoplasts at S3 and S5 developmental stages to assess the presence of anthocyanin biosynthetic enzymes and determine the suitability of flowers in different developmental stages as material for transient expression of TurboID-tagged bait proteins for proximity labeling.

2024-09-11 | PXD055749 | Pride

Proximity labeling of trans-cinnamic acid 4-hydroxylase in Petunia inflata petal protoplasts

Project description:Proximity-dependent labeling allows untargeted determination of both direct and indirect protein interactions in vivo, and therefore stands as an attractive alternative to targeted binary assays for determining global proteinprotein interaction networks. We used TurboID-based proximity labeling to study protein interaction networks of the core phenylpropanoid and anthocyanin pathways in petunia. To do so, we coupled the endoplasmic reticulum (ER) membrane anchored cytochrome P450 cinnamic acid 4-hydroxylase (C4H, CYP73A412) from Petunia inflata to TurboID and expressed it in protoplasts derived from anthocyanin-rich petunia petals. We identified multiple soluble enzymes from the late anthocyanin pathway among enriched proteins, along with other C4H isoforms, and other ER membrane anchored CYPs. Several of these interactions were subsequently confirmed by bimolecular fluorescence complementation (BiFC).

2024-09-11 | PXD055778 | Pride

Petunia ODO1 ChIP-seq and RNA-seq

Project description:Petunia floral scent production and emission is highly regulated, with a major role for the transcription factor ODORANT1 (ODO1) in directing activation of volatile biosynthesis. Using ChIP-seq of tagged ODO1 protein from petunia flowers, and RNA-seq of wild-type and odo1i RNAi flowers, the ODORANT1-regulated gene network of petunia is described, which extends to branches involved in phenylpropanoid intermediate production and S-adenosyl-methionine biosynthesis to potentiate production and emission of volatiles. Analysis of direct targets of regulation has also enabled the identification of an ODO1 binding motif.

2021-11-22 | GSE189280 | GEO

Revealing impaired pathways in regulatory mutant by high-throughput characterization of Petunia axillaris and Petunia inflata transcriptomes.

Project description:Petunia is an excellent model system, especially for genetic, physiological and molecular studies. Thus far, however, genome-wide expression analysis has been rarely applied because of the lack of sequence information. We applied next-generation sequencing to generate, through de novo read assembly, a large catalogue of transcripts for Petunia axillaris and Petunia inflata. On the basis of the transcriptome of each species, comprehensive microarray chips for gene expression analysis were established and used for the analysis of global- and organ-specific gene expression in both species. In addition, microarray analysis was applied to explore the molecular basis of the seed coat defects in Petunia hybrida mutants, homozygous for a null allele of the AN11 gene, encoding a WDR transcription regulator. Among the transcripts differentially expressed in an11 seeds compared to wild type, many expected targets of AN11 were found but also several interesting new candidates that might play a role in morphogenesis of the seed coat. Our results validate the combination of next-generation sequencing with microarray analyses strategies to identify the transcriptome of two petunia species without previous knowledge of their genome, and to develop comprehensive chips as useful tools for the analysis of gene expression in P. axillaris, P. inflata and P. hybrida.

2012-02-29 | GSE28120 | GEO

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