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Expression data of A224 cell with ectopic overexpression of FGF18 and RFP

ABSTRACT: We have identified the overexpression of FGF18 as an independent predictive marker for poor clinical outcome in patients with advanced stage, high-grade serous ovarian cancer. Functional studies have demonstrated that FGF18 promotes migration, invasion and tumorigenicity of ovarian cancer cells in vitro and in vivo. To identify the FGF18 responsive genes contributing its biologic effects on ovarian tumorigenesis, we performed gene expression profiling in ovarian cancer cell line A224 with ectopic overexpression of FGF18 or RFP (as control).

ORGANISM(S): Homo sapiens

PROVIDER: GSE48567 | GEO | 2013/07/08

SECONDARY ACCESSION(S): PRJNA210691

REPOSITORIES: GEO

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Expression data of A224 cell with ectopic overexpression of FGF18 and RFP

Project description:We have identified the overexpression of FGF18 as an independent predictive marker for poor clinical outcome in patients with advanced stage, high-grade serous ovarian cancer. Functional studies have demonstrated that FGF18 promotes migration, invasion and tumorigenicity of ovarian cancer cells in vitro and in vivo. To identify the FGF18 responsive genes contributing its biologic effects on ovarian tumorigenesis, we performed gene expression profiling in ovarian cancer cell line A224 with ectopic overexpression of FGF18 or RFP (as control). Microarrays were completed using total genomic DNA free RNA extracted from three independent paired cultures of A224 cells overexpressing FGF18 or RFP.

2013-07-08 | E-GEOD-48567 | biostudies-arrayexpress

Fgf18 regulates hair cycle

Project description:Fgf18 gene is strongly expressed in hair follicles of mouse dorsal skin during regressing (catagen) and resting (telogen) phases of hair cycle, but not in growth (anagen) phase. This study aims at identifying the function of Fgf18 in the regulation of hair cycle. To define target genes of Fgf18 during telogen phase of hair cycle, we generated mice in which Fgf18 gene is conditionally knocked out in keratin 5-positive epithelial cells (referred to as Fgf18 cKO below). We carried out microarray experiments with mouse back skin samples harboring telogen hair follicles obtained from three 42-d-old Fgf18 cKO male mice, or from three 42-d-old C57BL/6 male mice as control. Total RNA was isolated from each mouse and further purified to polyA RNA using oligo dT30 columns. The RNA samples were pooled for each group. Gene expression was analyzed by one-color analysis using duplicate arrays for each group.

2017-05-15 | E-GEOD-29547 | biostudies-arrayexpress

FGF18 affects hair cycle.

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2017-05-15 | E-GEOD-29546 | biostudies-arrayexpress

Fgf18 regulates hair cycle

2017-05-15 | GSE29547 | GEO

FGF18 affects hair cycle.

2017-05-15 | GSE29546 | GEO

Sorted MDMs with RFP+GFP+ or RFP+GFP- Mtb

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Characterization of aldehyde dehydrogenase 1 high ovarian cancer cells: Towards targeted stem cell therapy

Project description:Objective: The cancer stem cell (CSC) paradigm hypothesizes that successful clinical eradication of CSCs may lead to durable remission for patients with ovarian cancer. Despite mounting evidence in support of ovarian CSCs, their phenotype and clinical relevance remain unclear. We and others have found high aldehyde dehydrogenase 1 (ALDHhigh) expression in a variety of normal and malignant stem cells, and sought to better characterize ALDHhigh cells in ovarian cancer. Methods: We compared ALDHhigh to ALDHlow cells in two ovarian cancer models representing distinct subtypes: FNAR-C1 cells, derived from a spontaneous rat endometrioid carcinoma, and the human SKOV3 cell line (described as both serous and clear cell subtypes). We assessed these populations for stem cell features then analyzed expression by microarray and qPCR. Results: ALDHhigh cells displayed CSC properties, including: smaller size, quiescence, regenerating the phenotypic diversity of the cell lines in vitro, lack of contact inhibition, nonadherent growth, multi-drug resistance, and in vivo tumorigenicity. Microarray and qPCR analysis of the expression of markers reported by others to enrich for ovarian CSCs revealed that ALDHhigh cells of both models showed downregulation of CD24, but inconsistent expression of CD44, KIT and CD133. However, the following drugable targets were consistently expressed in the ALDHhigh cells from both models: mTOR signaling, her-2/neu, CD47 and FGF18 / FGFR3. Conclusions: Based on functional characterization, ALDHhigh ovarian cancer cells represent an ovarian CSC population. Differential gene expression identified drugable targets that have the potential for therapeutic efficacy against ovarian CSCs from multiple subtypes. FNAR-C1 rat ovarian cancer cells were sorted into Aldefluor high and Aldefluor low populations. Analysis was performed in triplicate. Aldefluor low cells served as the reference cells for fold-change analysis.

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Bovine granulosa cell response to FGF18

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expression data in lung of mice bearing inducible FGF18 transgene

Project description:Better understanding alveolarization mechanisms could help improving prevention and treatment of diseases characterized by reduced alveolar number, especially bronchopulmonary dysplasia (BPD). Although signaling through fibroblast growth factor (FGF) receptors is essential for alveolarization, involved ligands are unidentified. FGF18 whose expression peaks during alveolar septation is likely to be involved. Herein, a mouse model of inducible, lung-targeted FGF18-transgene was used to advance the onset of FGF18 expression, and genome-wide expression changes were determined. keywords: transgenic mice, alveolarization, angiogenesis, fibrosis, epithelial-mesenchymal transition. Four pairs of simple and double transgenic pups issued from 4 different litters were selected on the basis of FGF18 transgene induction. RNA from the mice lung were extracted and hybridized on Affymetrix microarrays.

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