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Differentially expressed genes associated with hepatic steatosis in zebrafish

ABSTRACT: Expression microarray of livers from adult zebrafish: control, overfed, and ahcy+/- Comparison of expression from livers obtained from ahcy+/+, overfed ahcy+/+, ahcy+/- control and ahcy+/- overfed.

ORGANISM(S): Danio rerio

PROVIDER: GSE48806 | GEO | 2013/10/01

SECONDARY ACCESSION(S): PRJNA211818

REPOSITORIES: GEO

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Differentially expressed genes associated with hepatic steatosis in zebrafish

Project description:Expression microarray of livers from adult zebrafish: control, overfed, and ahcy+/- Comparison of expression from livers obtained from ahcy+/+, overfed ahcy+/+, ahcy+/- control and ahcy+/- overfed. Adult zebrafish, ahcy+/+ and +/-, were given a regular diet or were overfed for a month. Livers were removed, RNA isolated, and expression microarray was performed.

2013-10-01 | E-GEOD-48806 | biostudies-arrayexpress

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Project description:Background: Obesity is a worldwide public health problem with increasing prevalence and affects 80% of diabetes mellitus type 2 cases. Zebrafish (Danio rerio) are an established model organism for studying obesity and diabetes including diabetic microvascular complications. We aimed to determine whether physical activity is an appropriate tool to examine training effects in zebrafish and to analyse metabolic and transcriptional processes in trained zebrafish. Methods: A 2- and 8-weeks experimental training phase protocol with adult zebrafish in a swim tunnel system was established. We examined zebrafish basic characteristics before and after training such as body weight, body length and maximum speed and considered overfeeding as an additional parameter in the 8-weeks training protocol. Ultimately, the effects of training and overfeeding on blood glucose, muscle core metabolism and liver gene expression using RNA-Seq were investigated. Results: Zebrafish maximum speed was correlated with body length and was significantly increased after 2 weeks of training. Maximum swim speed further increased after 8 weeks of training in both the normalfed and the overfed groups, but training was found not to be sufficient in preventing weight gain in overfed fish. Metabolome and transcriptome profiling in trained fish exhibited increased blood glucose levels in the short-term and upregulated energy supply pathways in the long-term. Conclusion: Swim training is a valuable tool to study effects of physical activity in zebrafish, which is accompanied by metabolic and transcriptional adaptations.

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Lack of de novo phosphatidylinositol synthesis leads to endoplasmic reticulum stress and hepatic steatosis in cdipt-deficient zebrafish

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