Unraveling the molecular basis of temperature-dependent genetic regulation in Penicillium marneffei
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ABSTRACT:
ORGANISM(S): Talaromyces marneffei PM1
PROVIDER: GSE48898 | GEO | 2013/07/20
SECONDARY ACCESSION(S): PRJNA212740
REPOSITORIES: GEO
Similar Datasets
Project description:Comparative analysis of transcriptome of Penicillium marneffei PM1 grown at 25°C and 37°C Penicillium marneffei strain PM1 was pre-cultured at 25°C and 37°C for two weeks on Sabouraud's Dextrose Agar (SDA). Messenger RNAs were then isolated from one-week 25°C and 37°C cultures and sequenced with Illumina by BGI America. Two replicates.
2013-07-20 | E-GEOD-48898 | biostudies-arrayexpress
Project description:Penicillium marneffei (Talaromyces marneffei) is an opportunistic human pathogen that can grow in a multicellular hyphal form at 25°C or a unicellular fission yeast form at 37°C, and can switch between these two forms in response to temperature. The yeast form is found in infected individuals and represents the pathogenic phase. In response to specific environmental cues, the hyphal form can undergo asexual development (conidiation) the produce a differentiated multicellular structure called a conidiophore which produces dormant spores (conidia). Inhaled conidia initiate infection. To identify genes that are important during the early stages in the transition from hyphal to yeast and yeast to hyphal cells transcriptional profiling was performed with a custom microarray consisting of ~42% of the predicted P. marneffei genes and RNA from P. marneffei hyphal cells switched to growth at 37°C for 6 hours (hyphal-yeast switch) and yeast cells switched to growth at 25°C for 6 hours (yeast-hyphal switch).
2013-11-21 | GSE51110 | GEO
Project description:Penicillium marneffei (Talaromyces marneffei) is an opportunistic human pathogen that can grow in a multicellular hyphal form at 25°C or a unicellular fission yeast form at 37°C, and can switch between these two forms in response to temperature. The yeast form is found in infected individuals and represents the pathogenic phase. In response to specific environmental cues, the hyphal form can undergo asexual development (conidiation) the produce a differentiated multicellular structure called a conidiophore which produces dormant spores (conidia). Inhaled conidia initiate infection. To identify genes that are ‘phase or cell-state specific’ transcriptional profiling was performed with a custom microarray consisting of ~42% of the predicted P. marneffei genes and RNA from P. marneffei hyphal, yeast and conidiation cell types.
2013-11-21 | GSE51109 | GEO
Project description:Penicillium marneffei is an opportunistic fungal pathogen endemic in Southeast Asia, causing lethal systemic infections in immunocompromised patients. P. marneffei grows in a mycelial form at the ambient temperature of 25°C and transitions to a yeast form at 37°C. The ability to alternate between the mycelial and yeast forms at different temperatures, namely, thermal dimorphism, has long been considered critical for the pathogenicity of P. marneffei, yet the underlying genetic mechanisms remain elusive. Here we employed high-throughput sequencing to unravel global transcriptional profiles of P. marneffei PM1 grown at 25 and 37°C. Among ∼11,000 protein-coding genes, 1,447 were overexpressed and 1,414 were underexpressed at 37°C. Counterintuitively, heat-responsive genes, predicted in P. marneffei through sequence comparison, did not tend to be overexpressed at 37°C. These results suggest that P. marneffei may take a distinct strategy of genetic regulation at the elevated temperature; the current knowledge concerning fungal heat response, based on studies of model fungal organisms, may not be applicable to P. marneffei. Our results further showed that the tandem repeat sequences (TRSs) are overrepresented in coding regions of P. marneffei genes, and TRS-containing genes tend to be overexpressed at 37°C. Furthermore, genomic sequences and expression data were integrated to characterize gene clusters, multigene families, and species-specific genes of P. marneffei. In sum, we present an integrated analysis and a comprehensive resource toward a better understanding of temperature-dependent genetic regulation in P. marneffei.
| S-EPMC3811563 | biostudies-literature
Project description:Penicillium marneffei (Talaromyces marneffei) is an opportunistic human pathogen that can grow in a multicellular hyphal form at 25M-BM-0C or a unicellular fission yeast form at 37M-BM-0C, and can switch between these two forms in response to temperature. The yeast form is found in infected individuals and represents the pathogenic phase. In response to specific environmental cues, the hyphal form can undergo asexual development (conidiation) the produce a differentiated multicellular structure called a conidiophore which produces dormant spores (conidia). Inhaled conidia initiate infection. To identify genes that are important during the early stages in the transition from hyphal to yeast and yeast to hyphal cells transcriptional profiling was performed with a custom microarray consisting of ~42% of the predicted P. marneffei genes and RNA from P. marneffei hyphal cells switched to growth at 37M-BM-0C for 6 hours (hyphal-yeast switch) and yeast cells switched to growth at 25M-BM-0C for 6 hours (yeast-hyphal switch). A custom microarray consisting of short, random genomic fragments from P. marneffei (~42% of the predicted genes) was generated using PCR products of the inserts from two independent DNA libraries constructed from genomic DNA of the P. marneffei type strain FRR2161. PCR products from previously cloned P. marneffei genes with known expression profiles were included as controls on the microarray. Total RNA from hyphal cells before and after switching to growth at 37M-BM-0C for 6 hours (hyphal-yeast switch) and yeast cells before and after switching to growth at 25M-BM-0C for 6 hours (yeast-hyphal switch) were used in pairwise combinations on the microarrays. Three biological replicate cultures were prepared for each experiment.
2013-11-21 | E-GEOD-51110 | biostudies-arrayexpress
Project description:Penicillium marneffei (Talaromyces marneffei) is an opportunistic human pathogen that can grow in a multicellular hyphal form at 25M-BM-0C or a unicellular fission yeast form at 37M-BM-0C, and can switch between these two forms in response to temperature. The yeast form is found in infected individuals and represents the pathogenic phase. In response to specific environmental cues, the hyphal form can undergo asexual development (conidiation) the produce a differentiated multicellular structure called a conidiophore which produces dormant spores (conidia). Inhaled conidia initiate infection. To identify genes that are M-bM-^@M-^Xphase or cell-state specificM-bM-^@M-^Y transcriptional profiling was performed with a custom microarray consisting of ~42% of the predicted P. marneffei genes and RNA from P. marneffei hyphal, yeast and conidiation cell types. A custom microarray consisting of short, random genomic fragments from P. marneffei (~42% of the predicted genes) was generated using PCR products of the inserts from two independent DNA libraries constructed from genomic DNA of the P. marneffei type strain FRR2161. PCR products from previously cloned P. marneffei genes with known expression profiles were included as controls on the microarray. Total RNA from hyphal, yeast or conidiation cell types was prepared and used in pairwise combinations (conidiation versus hyphal cells, conidiation versus yeast cells and hyphal versus yeast cells) on the microarrays. Three biological replicate cultures were prepared for each experiment.
2013-11-21 | E-GEOD-51109 | biostudies-arrayexpress
Project description:Draft Genome Sequence of Penicillium marneffei PM1
| PRJNA72349 | ENA
Project description:Penicillium marneffei Small RNA Transcriptome (17-30nt)
| PRJNA207279 | ENA
Project description:Penicillium marneffei: Gene expression profiling during the dimorphic switch
| PRJNA221202 | ENA
Project description:To investigate the function of NCOR2-013 in T. marneffei-infected THP-1 macrophages, we established NCOR2-013 overexpression THP-1 macrphages. We then performed gene expression profiling analysis using data obtained from RNA-seq of T. marneffei-infected NCOR2-013 overexpression THP-1 macrophages and T. marneffei-infected control cells.
2023-09-25 | GSE200512 | GEO