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Genome-wide DNA methylation maps during dehydration in leaf tissues of Boea hygrometrica

ABSTRACT: To examine the Boea hygrometrica genome methylation landscape and assess its functional significance during dehydration, we generated the first single-base resolution genome methylation maps for leaf tissues of hydrated, dehydrating to 70% RWC and dehydrating to 10% RWC (desiccating). The overall density of methyl-cytosine was not broadly altered by dehydration. There is, however, a small increase in intensity both for the whole genome and regionally during the first dehydration step (70% RWC) returning to near hydrated levels as leaves desiccate. We identified 5575 differentially methylated region-related genes during dehydration. The finding may provide an important clue for exploring molecular mechanisms and the regulation of desiccation tolerance at the whole-genome level.

ORGANISM(S): Dorcoceras hygrometricum

PROVIDER: GSE48904 | GEO | 2016/07/13

SECONDARY ACCESSION(S): PRJNA212240

REPOSITORIES: GEO

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Project description:Pre-exposure of plants to various abiotic conditions confers improved tolerance to subsequent stress. Mild drought acclimation induces acquired rapid desiccation tolerance (RDT) in the resurrection plant Boea hygrometrica, but the mechanisms underlying the priming and memory processes remain unclear. In this study, we demonstrated that drought acclimationinduced RDT can be maintained for at least four weeks but was completely erased after 18 weeks based on a combination of the phenotypic and physiological parameters. Global transcriptome analysis identified several RDT-specific rapid dehydration-responsive genes related to cytokinin and phospholipid biosynthesis, nitrogen and carbon metabolism, and epidermal morphogenesis, most of which were pre-induced by drought acclimation. Comparison of whole-genome DNA methylation revealed dehydration stress-responsive hypomethylation in the CG, CHG, and CHH contexts and acclimation-induced hypermethylation in the CHH context of the B. hygrometrica genome, consistent with the transcriptional changes in methylation pathway genes. As expected, the global promoter and gene body methylation levels were negatively correlated with gene expression levels in both acclimated and dehydrated plants but showed no association with transcriptional divergence during the procedure. Nevertheless, the promoter methylation variations in the CG and CHG contexts were significantly associated with the differential expression of genes required for fundamental genetic processes of DNA conformation, RNA splicing, translation, and post-translational protein modification during acclimation, growth, and rapid dehydration stress response. It was also associated with the dehydration stress-induced upregulation of memory genes, including pre-mRNA-splicing factor 38A, vacuolar amino acid transporter 1-like, and UDP-sugar pyrophosphorylase, which may contribute directly or indirectly to the improvement of dehydration tolerance in B. hygrometrica plants. Altogether, our findings demonstrate the potential implications of DNA methylation in dehydration stress memory and, therefore, provide a molecular basis for enhanced dehydration tolerance in plants induced by drought acclimation.

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Genome-wide DNA methylation maps during dehydration in leaf tissues of Boea hygrometrica

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